Lab Med Online.  2012 Jan;2(1):55-58. 10.3343/lmo.2012.2.1.10.

Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae

Affiliations
  • 1Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea. sjbjang@chosun.ac.kr
  • 2Research Center for Resistant Cells, Chosun University, Gwangju, Korea.
  • 3Department of Diagnostic Radiology, Chosun University College of Medicine, Gwangju, Korea.

Abstract

The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.

Keyword

Extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae; MicroScan; Phoenix; Double-disk synergy test
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