J Korean Med Sci.  2013 Nov;28(11):1697-1699. 10.3346/jkms.2013.28.11.1697.

Comparison of Specific IgE Antibodies to Wheat Component Allergens in Two Phenotypes of Wheat Allergy

  • 1Department of Internal Medicine, Dong-A University School of Medicine, Busan, Korea.
  • 2Department of Allergy & Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@ajou.ac.kr
  • 3Department of Internal medicine, Yeungnam University College of Medicine, Daegu, Korea.
  • 4Department of Pediatrics, Ajou University School of Medicine, Suwon, Korea.
  • 5Unidad de Bioquimica, Departamento de Biotecnologia, E.T.S. Ingenieros Agronomos, Madrid, Spain.


Specific IgE to gliadin was proposed as a marker for wheat dependent exercise induced anaphylaxis, while Tri a 14 was found to induce IgE response in baker's asthma. We evaluated whether these components could be used for discriminating phenotypes of wheat allergy. Twenty-nine patients who were wheat-induced anaphylaxis and/or urticaria (n=21, group I) and baker's asthma (n=8, group II) were enrolled. The prevalence of serum specific IgE to Tri a 14 was higher in group II (25%) than in group I (4.8%), while the serum specific IgE to gliadin was significantly higher in group I (70%) than in group II (12.5%). The cutoff value for predicting the baker's asthma using the ratio of serum specific IgE to Tri a 14 to gliadin was 742.8 optical densityx1,000/(kU/L) with high sensitivity and specificity. These findings suggest that Tri a 14/gliadin may be a potential marker for predicting baker's asthma.


Baker's Asthma; Gliadin; Lipid Transfer Protein; Wheat

MeSH Terms

Antigens, Plant/*immunology
Biological Markers/blood
Carrier Proteins/*immunology
Immunoglobulin E/*blood/immunology
Wheat Hypersensitivity/*diagnosis/*immunology
Antigens, Plant
Biological Markers
Carrier Proteins
Immunoglobulin E


  • Fig. 1 Receiver operating characteristic (ROC) curves for the ratio of serum specific IgE to Tri a 14 to gliadin. The area under ROC curve = 0.924.


1. Zuidmeer L, Goldhahn K, Rona RJ, Gislason D, Madsen C, Summers C, Sodergren E, Dahlstrom J, Lindner T, Sigurdardottir ST, et al. The prevalence of plant food allergies: a systematic review. J Allergy Clin Immunol. 2008; 121:1210–1218.e4.
2. Bock SA. Prospective appraisal of complaints of adverse reactions to foods in children during the first 3 years of life. Pediatrics. 1987; 79:683–688.
3. Wang J, Sampson HA. Food allergy: recent advances in pathophysiology and treatment. Allergy Asthma Immunol Res. 2009; 1:19–29.
4. Salcedo G, Quirce S, Diaz-Perales A. Wheat allergens associated with Baker's asthma. J Investig Allergol Clin Immunol. 2011; 21:81–92.
5. Matsuo H, Dahlström J, Tanaka A, Kohno K, Takahashi H, Furumura M, Morita E. Sensitivity and specificity of recombinant omega-5 gliadin-specific IgE measurement for the diagnosis of wheat-dependent exercise-induced anaphylaxis. Allergy. 2008; 63:233–236.
6. Bittner C, Grassau B, Frenzel K, Baur X. Identification of wheat gliadins as an allergen family related to baker's asthma. J Allergy Clin Immunol. 2008; 121:744–749.
7. Palacin A, Quirce S, Armentia A, Fernández-Nieto M, Pacios LF, Asensio T, Sastre J, Diaz-Perales A, Salcedo G. Wheat lipid transfer protein is a major allergen associated with baker's asthma. J Allergy Clin Immunol. 2007; 120:1132–1138.
8. Palacin A, Varela J, Quirce S, del Pozo V, Tordesillas L, Barranco P, Fernandez-Nieto M, Sastre J, Diaz-Perales A, Salcedo G. Recombinant lipid transfer protein Tri a 14: a novel heat and proteolytic resistant tool for the diagnosis of baker's asthma. Clin Exp Allergy. 2009; 39:1267–1276.
9. Houba R, Doekes G, Heederik D. Occupational respiratory allergy in bakery workers: a review of the literature. Am J Ind Med. 1998; 34:529–546.
10. Sander I, Merget R, Degens PO, Goldscheid N, Brüning T, Raulf-Heimsoth M. Comparison of wheat and rye flour skin prick test solutions for diagnosis of baker's asthma. Allergy. 2004; 59:95–98.
11. Mittag D, Niggemann B, Sander I, Reese I, Fiedler EM, Worm M, Vieths S, Reese G. Immunoglobulin E-reactivity of wheat-allergic subjects (baker's asthma, food allergy, wheat-dependent, exercise-induced anaphylaxis) to wheat protein fractions with different solubility and digestibility. Mol Nutr Food Res. 2004; 48:380–389.
12. Park HJ, Kim JH, Kim JE, Jin HJ, Choi GS, Ye YM, Park HS. Diagnostic value of the serum-specific IgE ratio of ω-5 gliadin to wheat in adult patients with wheat-induced anaphylaxis. Int Arch Allergy Immunol. 2012; 157:147–150.
13. Salcedo G, Sanchez-Monge R, Diaz-Perales A, Garcia-Casado G, Barber D. Plant non-specific lipid transfer proteins as food and pollen allergens. Clin Exp Allergy. 2004; 34:1336–1341.
14. Van Ree R. Clinical importance of non-specific lipid transfer proteins as food allergens. Biochem Soc Trans. 2002; 30:910–913.
15. Pastorello EA, Farioli L, Conti A, Pravettoni V, Bonomi S, Iametti S, Fortunato D, Scibilia J, Bindslev-Jensen C, Ballmer-Weber B, et al. Wheat IgE-mediated food allergy in European patients: alpha-amylase inhibitors, lipid transfer proteins and low-molecular-weight glutenins: allergenic molecules recognized by double-blind, placebo-controlled food challenge. Int Arch Allergy Immunol. 2007; 144:10–22.
16. Van Ree R, Chapman MD, Ferreira F, Vieths S, Bryan D, Cromwell O, Villalba M, Durham SR, Becker WM, Aalbers M, et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008; 63:310–326.
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