Korean J Vet Res.  2014 Sep;54(3):171-177.

Chemopreventive effects of garlic and mugwort mixture extract on Helicobacter pylori-associated mouse gastric carcinogenesis

• Youm SY
• Kim TW
• Shin SK
• Jeong HS
• Kang JK
• Kim TM
• Kim KH
• Ahn B

Abstract

Garlic and mugwort have long been used in traditional medicine to prevent various diseases. Several in vitro studies have reported protective efficacies of garlic and mugwort in cases of gastric cancer. In the present study, we investigated the cancer preventive effects of garlic and mugwort mixture extract (GME) in a Helicobacter (H.) pylori-associated gastric carcinogenesis mouse model. To induce gastric cancer, C57BL/6 mice were treated with N-methyl-N-nitrosourea and H. pylori. Various concentrations of GME (0, 100, 500, and 1,000 ppm) were then fed to the mice for 38 weeks, after which the tumor tissues were examined for histopathology, mucin histochemistry and beta-catenin. The incidence of gastric tumors was significantly lower in the highest dose GME-treated mice (46.7%) than control mice (85.7%) (p < 0.05). The multiplicity and size of tumors were also significantly reduced by GME feeding in a dose-dependent manner (p < 0.01). Furthermore, GME suppressed the H. pylori-associated chronic inflammation measured by histologic grading of H. pylori density, chronic gastritis, glandular atrophy and intestinal metaplasia in non-tumorous gastric mucosae. Our data suggest that GME suppresses gastric tumorigenesis via suppression of H. pylori-associated chronic inflammation.

Keyword

artemisia; garlic; gastric cancer; Helicobacter pylori; mouse

MeSH Terms

Animals
Artemisia*
Atrophy
beta Catenin
Carcinogenesis*
Garlic*
Gastric Mucosa
Gastritis
Helicobacter pylori
Helicobacter*
Incidence
Inflammation
Medicine, Traditional
Metaplasia
Methylnitrosourea
Mice*
Mucins
Stomach Neoplasms
Methylnitrosourea
Mucins
beta Catenin