Korean J Parasitol.  2014 Feb;52(1):9-19. 10.3347/kjp.2014.52.1.9.

Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia

Affiliations
  • 1Laboratory of Fish Parasites and Diseases, Department of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia. hilalanshary@gmail.com, hilalanshary@unhas.ac.id
  • 2Institute of Ocean and Earth Sciences, University of Malaya, Malaysia.
  • 3Meguro Parasitological Museum, Meguro-ku, Tokyo 153-0064, Japan.

Abstract

Anisakis spp. (Nematoda: Anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. In this study, 18 fish species were investigated for Anisakis infection. Katsuwonus pelamis, Euthynnus affinis, Caranx sp., and Auxis thazard were infected with high prevalence of Anisakis type I, while Cephalopholis cyanostigma and Rastrelliger kanagurta revealed low prevalence. The mean intensity of Anisakis larvae in K. pelamis and A. thazard was 49.7 and 5.6, respectively. A total of 73 Anisakis type I larvae collected from K. pelamis and A. thazard were all identified as Anisakis typica by PCR-RFLP analysis. Five specimens of Anisakis from K. pelamis and 15 specimens from A. thazard were sequenced using ITS1-5.8S-ITS2 region and 6 specimens from A. thazard and 4 specimens from K. pelamis were sequenced in mtDNA cox2 region. Alignments of the samples in the ITS region showed 2 patterns of nucleotides. The first pattern (genotype) of Anisakis from A. thazard had 100% similarity with adult A. typica from dolphins from USA, whereas the second genotype from A. thazard and K. pelamis had 4 base pairs different in ITS1 region with adult A. typica from USA. In the mtDNA cox2 regions, Anisakis type I specimens from A. thazard and K. pelamis showed similarity range from 94% to 99% with A. typica AB517571/DQ116427. The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait.

Keyword

Anisakis typica; molecular identification; internal transcribed spacer; PCR-RFLP; Makassar Strait; Indonesia

MeSH Terms

Animals
Anisakiasis/epidemiology/parasitology/*veterinary
Anisakis/*isolation & purification
Cluster Analysis
DNA Fingerprinting
DNA, Intergenic/chemistry/genetics
Fish Diseases/*epidemiology/*parasitology
Genotype
Indonesia/epidemiology
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prevalence
RNA, Ribosomal, 5.8S/genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
DNA, Intergenic
RNA, Ribosomal, 5.8S
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