Korean J Parasitol.  2014 Aug;52(4):383-389. 10.3347/kjp.2014.52.4.383.

Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

• Sohn WM
• Kang JM
• Na BK

• KMID: 1792199
• DOI: http://doi.org/10.3347/kjp.2014.52.4.383

Abstract

Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.

Keyword

Anisakis; PCR-RFLP; ITS; cox1

MeSH Terms

Animals
Anisakiasis/parasitology/*veterinary
Anisakis/*classification/genetics/*isolation & purification
Aquatic Organisms
Cluster Analysis
DNA, Helminth/chemistry/genetics
DNA, Ribosomal Spacer/chemistry/genetics
Electron Transport Complex IV/genetics
Fish Diseases/*parasitology
Fishes
Korea
Larva/classification/genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
DNA, Helminth
DNA, Ribosomal Spacer
Electron Transport Complex IV