Ann Lab Med.  2013 Nov;33(6):426-430. 10.3343/alm.2013.33.6.426.

Frequency of FCGR3B Alleles in Thai Blood Donors

• Kaset C
• Leetrakool N
• Intharanut K
• Nathalang

• KMID: 1711316
• DOI: http://doi.org/10.3343/alm.2013.33.6.426

Abstract

BACKGROUND
Human neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations.
METHODS
Eight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique.
RESULTS
The frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the FCGR3B*2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations.
CONCLUSIONS
FCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.

Keyword

FCGR3B; Allele frequencies; Thais

MeSH Terms

Asian Continental Ancestry Group/*genetics
*Blood Donors
DNA/analysis
DNA Primers/chemistry/metabolism
GPI-Linked Proteins/genetics
Gene Frequency
Genotype
Humans
Polymerase Chain Reaction
Receptors, IgG/*genetics
Thailand
DNA
DNA Primers
GPI-Linked Proteins
Receptors, IgG

Figure

• 

  Fig. 1 PCR sequence-specific primer analysis of the FCGR3B*1-, FCGR3B*2-, and FCGR3B*3-positive individuals. The 141-bp, 219-bp, and 191-bp bands represent the specific PCR products and the 434-bp HGH bands are the internal controls. PCR products were analyzed on a 2.0% agarose gel stained with ethidium bromide. The DNA size standard (100-bp ladder marker; Fermentas, Carlsbad, CA, USA) is shown in lane M.

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