- Detection of Bacillus anthracis using a nested PCR Method
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Choi YK, Cho SK, Kim MH, Baik SY, Park GH, Chai YG
- KMID: 2146918
- J Korean Soc Microbiol.
- 1998 Dec;33(6):583-588.
Bacillus anthracis is a soil pathogen capable of causing anthrax in animals and humans. To establish a method for specifically detecting B. anthracis, we used nested polymerase chain reaction. Outer... -
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- Development of a Rapid Detection Method for Yersinia pestis by Polymerase Chain Reaction
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Oh HJ, Min HK, Sohn YW, Chun JH, Park HO
- KMID: 2146898
- J Korean Soc Microbiol.
- 1999 Aug;34(4):373-383.
A polymerase chain reaction (PCR) method for detection of the pathogenic Yersinia pestis from other Yersinia spp. was developed. Five Y. pestis strains, ninety-two other Yersinia species and twenty-four Enterobacteriaceae... -
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- The Diagnosis of Tuberculous Meningitis by Polymerase Chain Reaction
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Kim JW, Lee SK, Ha KM
- KMID: 1686121
- J Korean Neurol Assoc.
- 1992 Mar;10(1):8-13.
Polymerase chain reaction (PCR) method is a proced.ure for in vitro enzymatic ampli fication of a specific segment of DNA. PCR method was used to detect any M. tuberculi DNA... -
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- A Study of Genetic Polymorphisms of New HLA Genes, TAP1 and TAP2
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Lee KO, Heo JH, Hong SH
- KMID: 2083017
- Korean J Immunol.
- 1998 Mar;20(1):69-74.
The objective of this study is to establish the genotyping methods of new HLA gene, TAP1 and TAP2, and determine the genetic polymorphisms for database study in Koreans before using... -
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- Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type
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Yu JR, Chung JS, Chai JY
- KMID: 1715318
- Korean J Parasitol.
- 1997 Dec;35(4):295-298.
- doi: 10.3347/kjp.1997.35.4.295
Genomic DNA from Metagonimus yokogawai and Metagonimus Miyata type was amplified by polymerase chain reaction based on the random amplification of polymorphic DNA (RAPD) technique. Eight random 10-mer oligonucleotide... -
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- PCR in diagnosis of pneumocystosis of rats
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Hong ST
- KMID: 1715355
- Korean J Parasitol.
- 1996 Sep;34(3):191-196.
- doi: 10.3347/kjp.1996.34.3.191
Polymerase chain reaction (PCR) is a powerful technique to detect scanty amount of DNA from living organisms. The present study intended to develop specific primers for PCR diagnosis of pneumocystosis... -
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- Detection of BLV Proviral DNA in Korean Native Goats Experimentally Infected with Bovine Leukemia Virus by Polymerase Chain Reaction
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Jun MH, Chang KS, Cho YS, Park JH, An SH
- KMID: 1541349
- J Korean Soc Virol.
- 1997 Dec;27(2):217-226.
PCR implication using the primers for gag, pol and rev genes in BLV (bovine leukemia virus) proviral DNA and syncytium assay were carried out for the Korean native goats experimentally... -
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- Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb
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Oh CS, Seo M, Hong JH, Chai JY, Oh SW, Park JB, Shin DH
- KMID: 2381824
- Korean J Parasitol.
- 2015 Apr;53(2):237-242.
- doi: 10.3347/kjp.2015.53.2.237
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in... -
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- Bacterial Translocation and Prognosis of Critically Ill Patients
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Kim WW, Sung CK, Oh SH, Kim SH
- KMID: 2209464
- J Korean Surg Soc.
- 2002 Jun;62(6):472-479.
PURPOSE: To identify Bacterial translocation (BT) from the gut to the blood in the critically ill patients by using the polymerase chain reaction (PCR) to confirm the sensitivity of PCR... -
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- Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
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Chontananarth T, Wongsawad
- KMID: 1104704
- Korean J Parasitol.
- 2010 Dec;48(4):343-346.
- doi: 10.3347/kjp.2010.48.4.343
The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988... -
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- Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system
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Moon HJ, Park SJ, Kim HK, Ann SK, Rho S, Keum HO, Park BK
- KMID: 1093503
- J Vet Sci.
- 2010 Sep;11(3):269-271.
- doi: 10.4142/jvs.2010.11.3.269
The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV) proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a... -
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- Direct detection of cariogenic streptococci in metal brackets in vivo using polymerase chain reaction
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Ahn SJ, Lee SJ, Baek SH, Kim TW, Chang YI, Nahm DS, Lim BS
- KMID: 1762558
- Korean J Orthod.
- 2005 Aug;35(4):312-319.
Streptococcus mutans and Streptococcus sobrinus are major etiological agents in enamel demineralization around orthodontic appliances. This study was designed to examine the prevalence of these streptococci on orthodontic brackets in... -
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- Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR
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Suh DK, Lym SK, Bae YC, Lee KW, Choi WP, Song JC
- KMID: 1103472
- J Vet Sci.
- 2000 Jun;1(1):33-37.
Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE)... -
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- Detection of Thermostable Direct Hemolysin Gene and Thermostable Direct Hemolysin-Related Hemolysin Gene of Vibrio parahaemolyticus by Polymerase Chain Reaction
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Kim WJ, Keum DG, Soe YH, Choi TY
- KMID: 2146105
- Korean J Clin Pathol.
- 2001 Oct;21(5):355-359.
BACKGROUND: Vibrio parahaemolyticus is one of the most important food-borne pathogens in Korea. Although the mechanism of its pathologic effect is still not clearly understood, epidemiological studies have suggested a... -
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- Frepuency of the Angiotensin - Converting Enzyme (ACE) Gene Polymorphism in the General Population and the Elite Endurance Students in Korea
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Choung HJ, Yoon SR, Choi SK
- KMID: 1536937
- J Genet Med.
- 1999 Dec;3(1):11-14.
Recintly it was reported that Insertion / Deletion polymorphism in the gene coding for Angiotensin Converting Enzyme (ACE) is asscoiated with human capacity for physical performance. This study was performed... -
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- Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE
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Shin EK, Seo YS, Han JH, Hahn TW
- KMID: 1104238
- J Vet Sci.
- 2007 Mar;8(1):65-73.
- doi: 10.4142/jvs.2007.8.1.65
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41)... -
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- Detection of Helicobacter DNA in bile from bile duct diseases
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Roe IH, Kim JT, Lee HS, Lee JH
- KMID: 648078
- J Korean Med Sci.
- 1999 Apr;14(2):182-186.
- doi: 10.3346/jkms.1999.14.2.182
Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of... -
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- Polymorphism of Angiotensin Converting Enzyme in Hypertensive Intracerebral Hemorrhage Patients
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Kim DH, Kim CJ, Kim SK, Jho JW, Lee JC, Kim HI, Kho KY
- KMID: 2339605
- J Korean Neurosurg Soc.
- 1998 Apr;27(4):425-429.
Polymorphism in the angiotensin-converting enzyme(ACE) gene may confer an increased risk of vascular disease. DD type of ACE polymorphism predispose a person to myocardial infarction and IgA nephropathy. The roles... -
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- Hepatitis C virus genotyping of 100 consecutive anti-HCV positive cases with PCR using type=specific primers
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Lee YS, Chung YH, Min YI, Moon DH, Na DS, Suh DJ
- KMID: 1534900
- Korean J Hepatol.
- 1998 Sep;4(3):235-243.
BACKGROUND/AIMS: The distribut ion of HCV genotypes varies with geographical area, and genot ypes can affect t he nat ur al course of HCV infection, but adequate genot yping data were... -
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- Development of a multiplex PCR to identify Salmonella, Leptospira and Brucella species in tissue samples
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Lam TQ, Yoon BI, Hahn TW
- KMID: 1435817
- Korean J Vet Res.
- 2012 Jun;52(2):75-82.
We have developed and optimized a multiplex polymerase chain reaction (mPCR) for simultaneous detection of Brucella, Salmonella and Leptospira with high sensitivity and specificity. Three pairs of oligonucleotide primers were... -
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