J Korean Rheum Assoc.  2007 Jun;14(2):118-124. 10.4078/jkra.2007.14.2.118.

The Expression of Toll-like Receptors in Idiopathic Inflammatory Myopathies

• Kim GT
• Lee JH
• Baek SH
• Lee JW
• Cho MR
• Kim JI
• Lee SH
• Kim DS
• Kim SI

• KMID: 1526428
• DOI: http://doi.org/10.4078/jkra.2007.14.2.118

Abstract

Objective
To investigate the expression of toll-like receptor (TLR)-2, 4 and 9 in idiopathic inflammatory myopathies (IIMs).
Methods
The expression of TLR-2, 4 and 9 was measured by real-time RT-PCR and immunohistochemical stain (IHS) from muscle tissues in patients with IIMs and controls.
Results
The expression levels of TLR-2, 4 and 9 in IIMs were significantly higher than controls. TLR-2, 4 and 9 were mainly expressed on sarcolemma of muscle fibers, perimysial vascular endothelium and infiltrating inflammatory cells in dermatomyositis, whereas, they were mainly expressed on sarcolemma of muscle fibers, destructed muscle fibers, and enodmysial infiltrating inflammatory cells in polymyositis.
Conclusion
TLR-2, 4 and 9 were highly expressed in muscle tissue of IIMs. These results suggest that TLR-2, 4 and 9 play a role in pathogenesis of IIMs.

Keyword

Toll-like receptors; Idiopathic inflammatory myopathy

MeSH Terms

Dermatomyositis
Endothelium, Vascular
Humans
Myositis*
Polymyositis
Sarcolemma
Toll-Like Receptors*
Toll-Like Receptors

Figure

• 

  Fig. 1. Immunohistochemical stain of TLR-2 in muscle tissues from healthy control (A) & patients with IIMs (B, C). (B) In DM, TLR-2 is expressed by sarcolemma of muscle fibers, perimysial vascular endothelium and infiltrating inflammatory cells (arrow). (C) In PM, TLR-2 is expressed by sarcolemma of muscle fibers, destructed muscle fibers, and enodmysial infiltrating inflammatory cells (arrow). Magnification: ∗200.

• 

  Fig. 2. Immunohistochemical detection of TLR-4 in muscle tissues from healthy control (A) & patients with IIMs (B, C). (B) In DM, TLR-4 is expressed by sarcolemma of muscle fibers, perimysial vascular endothelium and infiltrating inflammatory cells (arrow). (C) In PM, TLR-4 is expressed by sarcolemma of muscle fibers, destructed muscle fibers, and enodmysial infiltrating inflammatory cells (arrow). Magnification: ∗200.

• 

  Fig. 3. Immunohistochemical detection of TLR-9 in muscle tissues from healthy control (A) & patients with IIMs (B, C). (B) In DM, TLR-9 is expressed by sarcolemma of muscle fibers, perimysial vascular endothelium and infiltrating inflammatory cells (arrow). (C) In PM, TLR-9 is expressed by sarcolemma of muscle fibers, destructed muscle fibers, and enodmysial infiltrating inflammatory cells (arrow). Magnification: ∗200.

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