Korean J Pediatr.  2008 Dec;51(12):1336-1341. 10.3345/kjp.2008.51.12.1336.

Distribution of CD4+CD25+ T cells and graft-versus-host disease in human hematopoietic stem cell transplantation

• Lee DH
• Chung NG
• Jeong DC
• Cho B
• Kim HK

• KMID: 1494525
• DOI: http://doi.org/10.3345/kjp.2008.51.12.1336

Abstract

PURPOSE: This study aimed to determine the frequencies of CD4+CD25+ T cells in donor graft and peripheral blood CD4+CD25+ T cells in recipients after hematopoietic stem cell transplantation (HSCT) and their association with graft-versus-host disease (GVHD).
METHODS
Seventeen children who underwent HSCT were investigated. CD4+CD25+ T cells in samples from donor grafts and recipient peripheral blood were assessed by flow cytometry at 1 and 3 months after transplantation.
RESULTS
CD4+CD25+ T cell frequencies in the grafts showed no significant difference between patients with and without acute GVHD (0.90% vs. 1.06%, P=0.62). Absolute CD4+CD25+ T cell number in grafts were lower in patients with acute GVHD than in those without acute GVHD (6.18x10(5)/kg vs. 25.85x10(5)/kg, P=0.09). Patients without acute GVHD showed a significant decrease in peripheral blood CD4+CD25+ T cell percentage at 3 months compared to those at 1 month after HSCT (2.11% vs. 1.43%, P=0.028). However, in patients with acute GVHD, CD4+CD25+ T cell percentage at 3 months was not different from the corresponding percentage at 1 month after HSCT (2.47% vs. 2.30%, P=0.5).
CONCLUSION
The effect of frequencies of CD4+CD25+ T cells in donor grafts on acute GVHD after HSCT could not be identified, and the majority of peripheral blood CD4+CD25+ T cells in patients who underwent HSCT may be activated T cells related to acute GVHD rather than regulatory T cells. Further studies with additional markers for regulatory T cells are needed to validate our results.

Keyword

Regulatory T cell; Graft-versus-fost Disease; Allogeneic hematopoietic stem cell transplantation

MeSH Terms

Cell Count
Child
Flow Cytometry
Graft vs Host Disease
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
T-Lymphocytes
T-Lymphocytes, Regulatory
Tissue Donors
Transplants