Tuberc Respir Dis.  2008 Jan;64(1):15-21. 10.4046/trd.2008.64.1.15.

The Utility of MAGE Gene Detection in Bronchial Washing Fluid for Patients with Peripheral NSCLC

• Kim S
• Kim H
• Kwon OJ
• Chung MP
• Suh GY
• Koh WJ
• Ham CR
• Nam HS
• Um SW
• Kwon YS
• Park S

• KMID: 1478136
• DOI: http://doi.org/10.4046/trd.2008.64.1.15

Abstract

BACKGROUND: The melanoma antigen-encoding (MAGE) genes are known to be expressed in various cancer cells, including non-small cell lung cancer (NSCLC), and are silent in all normal tissues except for the testis. In patients with peripheral NSCLC, bronchial washing fluid can be used to detect the MAGE genes, suggesting a diagnosis of lung cancer. In order to evaluate the diagnostic utility of the MAGE test in patients with peripheral NSCLC, bronchial washing fluid was investigated in patients with peripheral pulmonary nodules, which were invisible as detected by bronchoscopy.
METHODS
Bronchial washing fluid from 37 patients was used for cytological examinations and MAGE gene detection, using RT-nested-PCR of common A1-A6 mRNA. Results were compared to a final diagnosis of patients as confirmed by pathology.
RESULTS
Among the 37 subjects, NSCLC was diagnosed in 21 patients, and benign pulmonary diseases were diagnosed in 16 patients. MAGE mRNA was detected in 10 of 21 (47.6%) NSCLC patients, while conventional cytology examinations were positive for MAGE expression in 2 of 21 (9.5%) cases. MAGE expression was observed in 4 of 16 (25%) benign pulmonary disease patients.
CONCLUSION
The MAGE test of bronchial washing fluid can be used as a sensitive predictor of peripheral NSCLC patients.

Keyword

Melanoma antigen gene (MAGE); Lung neoplasm; Coin lesion

MeSH Terms

Bronchoscopy
Carcinoma, Non-Small-Cell Lung
Humans
Lung Diseases
Lung Neoplasms
Melanoma
RNA, Messenger
Testis
RNA, Messenger

Figure

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  Figure 1 Amplification of MAGE 1-6 by nested RT-PCR using common MAGE primers. Lane 1: Negative control, Lane 2: Tumor tissue, Lane 3: Prostate cancer cell line, Lane 4: Somatic cancer cell line, Lane 5: MAGE positive control, Lane 6: Sample 1, Lane 7: Sample 2, Lane 8: Normal tissue. GAPDH: glyceraldehydes phosphate dehydrogenase.

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