Lab Anim Res.  2010 Mar;26(1):7-13. 10.5625/lar.2010.26.1.7.

Anti-inflammatory Effect of Quercetin on Picryl Chloride-induced Contact Dermatitis in BALB/c Mice

• Kim HJ
• Kim J
• Kim SJ
• Lee SH
• Park YS
• Park BK
• Kim BS
• Kim SK
• Cho SD
• Jung JW
• Nam JS
• Choi C
• Jung JY

• KMID: 1458934
• DOI: http://doi.org/10.5625/lar.2010.26.1.7

Abstract

In this study, we investigated that anti-inflammatory effect of quercetin on picryl chloride(PCL)-induced contact dermatitis in BALB/c Mice. Experimental animals were divided into three groups and comprising five animals. All groups of oral administration was begun on the first day of PCL treatment and ceased on day 5. For the induction of contact dermatitis, BALB/c mice were sensitized with 40 microliter of 1.5% picryl choloride (PCL) to the left and right ear, respectively. Ear swelling responses were much weaker in high-dose group (100 mg/kg) than control group (0 mg/kg). Total serum IgE levels and histamine levels were measured by sandwich ELISA method using mouse IgE, histamine measuring Kit. Both total serum IgE and histamine levels were significantly decreased in high-dose group (100 mg/kg) than other groups. Degranulation of mast cells were also confirmed by Toluidine Blue (TB) staining method. In high-dose group (100 mg/kg), the number of mast cells were significantly decreased and there are many mast cells were shown degranulation in control group (0 mg/kg). All of these results demonstrate that the pharmacological actions of quercetin indicate their potential activity for allergic inflammatory diseases through the down-regulation of mast cell activation.

Keyword

Quercetin; picryl chloride; contact dermatitis; IgE; histamine

MeSH Terms

Administration, Oral
Animals
Dermatitis, Contact
Down-Regulation
Ear
Enzyme-Linked Immunosorbent Assay
Histamine
Immunoglobulin E
Mast Cells
Mice
Picryl Chloride
Quercetin
Tolonium Chloride
Histamine
Immunoglobulin E
Picryl Chloride
Quercetin
Tolonium Chloride

Figure

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  Figure 1. Ear swelling responses in BALB/c mice at 0, 3, 6, 9, 12, 18, 24 hr following the sensitization with PCL to the ear. Each value was expressed as Mean±SE of 5 BALB/c mice. ∗Significantly different from 0 mg/kg group at P<0.05.

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  Figure 2. Total serum IgE levels in female BALB/c mice at 3 hr after application with PCL. Each value was expressed as Mean±SE of 5 BALB/c mice. ∗Significantly different from 0 mg/ kg group at P<0.05.

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  Figure 3. Total serum Histamine levels in female BALB/c mice at 3 hr after application with PCL. Each value was expressed as Mean±SE of 5 BALB/c mice. ∗Significantly different from 0 mg/ kg group at P<0.05.

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  Figure 4. Ear skin of 0 mg/kg group (A, C) and 100 mg/kg quercetin group (B, D) in BALB/c mice at 3 hr after application with PCL. (A) Intradermal edema with prominent inflammatory cell infiltration. H&E, ×200. (B) Less severe changes are seen compared with (A). H&E, ×200. (C) There are many mast cells including those showing degranulation. TB ×200. (D) Mast cells show less degranulation compared with (C). TB ×200.

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  Figure 5. The number of mast cells in female BALB/c mice at 3 hr after application with PCL. Each value was expressed as Mean±SE of 5 BALB/c mice. ∗Significantly different from 0 mg/ kg group at P<0.05.

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