J Korean Assoc Oral Maxillofac Surg.  2010 Feb;36(1):1-6. 10.5125/jkaoms.2010.36.1.1.

Stromal cell-derived factor-1 (SDF-1) expression in the oral squamous cell carcinoma

• Kim KW
• Han SJ
• Roh KS

• KMID: 1457812
• DOI: http://doi.org/10.5125/jkaoms.2010.36.1.1

Abstract

PURPOSE
Chemokines are structurally related, small polypeptide signaling molecules that bind to and activate a family of transmembrane G proteincoupled receptors, the chemokine receptors. Recently, interaction between the chemokine receptor CXCR4 and its ligand, stromal cell-derived factor 1 (SDF-1 or CXCL12), has been found to play an important role in tumorigenicity, proliferation, metastasis and angiogenesis in many cancers such as lung cancer, breast cancer, melanoma, glioblastoma, pancreatic cancer and cholangiocarcinoma. Hence, the goal of this study is to identify the correlation of clinicopathological factors and the up-regulation of SDF-1 expression in oral squamous cell carcinoma. MATERIAL AND METHODS: We studied the immunohistochemical staining of SDF-1, quantitative RT-PCR (qRT-PCR) of SDF-1 gene in 20 specimens of 20 patients with oral squamous cell carcinoma.
RESULTS
1. In the immunohistochemical study of poor differentiated and invasive oral squamous cell carcinoma, the high level staining of SDF-1 was observed. And the correlation between immunohistochemical SDF-1 expression and tumor nodes metastases (TNM) classification of specimens was significant.(chi-square test, P < 0.05) 2. In the SDF-1 gene qRT-PCR analysis, SDF-1 expression was more in tumor tissue than in carcinoma in situ tissue. Paired-samples analysis determined the difference of SDF-1 mRNA expression level between the cancer tissue and the carcinoma in situ tissue.(Student's t-test, P < 0.05)
CONCLUSION
These findings suggest that up-regulation of the SDF-1 may play a role in progression and invasion of oral squamous cell carcinoma.

Keyword

Chemokine; Chemokine CXCL12 (SDF-1); Oral squamous cell carcinoma

MeSH Terms

Breast Neoplasms
Carcinoma in Situ
Carcinoma, Squamous Cell
Chemokine CXCL12
Chemokines
Cholangiocarcinoma
Glioblastoma
Humans
Lung Neoplasms
Melanoma
Neoplasm Metastasis
Pancreatic Neoplasms
Receptors, Chemokine
RNA, Messenger
Up-Regulation
Chemokine CXCL12
Chemokines
RNA, Messenger
Receptors, Chemokine

Figure

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  Fig. 1. Immunohistochemical staining for SDF-1 of normal oral tissue.(×400)

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  Fig. 2. Immunohistochemical staining for SDF-1 of oral carcinoma in situ.(×400)

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  Fig. 3. Immunohistochemical staining for SDF-1 of moderate differentiated oral squamous cell carcinoma.(×400)

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  Fig. 4. Immunohistochemical staining for SDF-1 of poor differentiated and invasive oral squamous cell carcinoma.(×400) (SDF-1: stromal cell-derived factor-1)

Reference

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