Lab Anim Res.  2012 Dec;28(4):239-244. 10.5625/lar.2012.28.4.239.

Early diagnosis of radiodermatitis using lactate dehydrogenase isozymes in hairless mice (SKH1-hr)

• Cho SK
• Kim WD

• KMID: 1431407
• DOI: http://doi.org/10.5625/lar.2012.28.4.239

Abstract

In this study, we evaluate a method for the early diagnosis of radiodermatitis for use in the prevention and therapy of this condition. Hairless mice (SKH1-hr) were used to study the early diagnosis of radiodermatitis. Lactate dehydrogenase (LDH, EC 1.1.1.27) isozymes were analyzed using native-polyacrylamide gel electrophoresis and western blotting of blood serum and tissues collected from SKH1-hr mice. Radiodermatitis developed 24 days after the first X-irradiation. Reduced spleen weight was observed after the last X-irradiation (P<0.05). Thereafter the weight increased until 24 days after the first irradiation, finally reaching levels comparable to those in the sham-irradiated control group. LDH activity was the highest in skeletal muscle and lowest in blood serum. LDH C4, A4, A3B, A2B2, AB3, and B4 isozymes were detected, in the mentioned order, from the cathode. This result was similar in other mouse strains. In the irradiated group, LDH A4 isozyme levels were reduced in the serum until inflammation occurred, whereas those of B4 isozyme were elevated. The subunits A and B followed a similar trend to that of LDH A4 and B4 isozyme, respectively. Importantly, antibodies against LDH B4 isozyme could prove useful in the early diagnosis of radiodermatitis.

Keyword

Radiodermatitis; hairless mice (SKH1-hr); lactate dehydrogenase (LDH); isozyme

MeSH Terms

Animals
Antibodies
Blotting, Western
Early Diagnosis
Electrodes
Electrophoresis
Inflammation
Isoenzymes
L-Lactate Dehydrogenase
Lactic Acid
Mice
Mice, Hairless
Muscle, Skeletal
Radiodermatitis
Serum
Spleen
Antibodies
Isoenzymes
L-Lactate Dehydrogenase
Lactic Acid

Figure

• 

  Figure 1 Macrophotography of the radiodermatitis-induced SKH1-hr mice. The arrow indicates the region with inflammation.

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  Figure 2 The weight of the spleen. C, Sham-irradiated control (○); X-irradiated (●). *Represents P<0.05 compared with the sham-irradiated control. Data are expressed as means±SD (n=4).

• 

  Figure 3 Native-polyacrylamide gel electrophoresis zymograms (a-g) and western blot analysis (h, i) of the lactate dehydrogenase (LDH) isozymes of SKH1-hr mice. Electrophoretic patterns of the skeletal muscle (a), heart (b), kidney (c), liver (d), spleen (e), testis (f), and blood serum (g). Western blotting of LDH A4 (h) and B4 isozyme (i). A4, LDH A4 isozyme; B4, LDH B4 isozyme; C4, LDH C4 isozyme; o, origin.

• 

  Figure 4 The relative expressions (%) of LDH subunit A (●), B (▼), and C (■) in the blood serum of the SKH1-hr mice with induced radiodermatitis. C, Sham-irradiated control. *Represents P<0.05 compared with the sham-irradiated control. Data are expressed as means±SD (n=4).

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