Int J Stem Cells.  2012 May;5(1):79-83.

In vivo Tracking of Human Neural Stem Cells Following Transplantation into a Rodent Model of Ischemic Stroke

• Chang DJ
• Moon H
• Lee YH
• Lee N
• Lee HJ
• Jeon I
• Lee H
• Hwang TS
• Oh SH
• Shin DA
• Kim SU
• Hong KS
• Song J

Abstract

BACKGROUND AND OBJECTIVES
Ischemic stroke caused by middle cerebral artery occlusion (MCAo) is the major type of stroke, but there are currently very limited options for cure. It has been shown that neural stem cells (NSCs) or neural precursor cells (NPCs) can survive and improve neurological deficits when they are engrafted in animal models of various neurological diseases. However, how the transplanted NSCs or NPCs are act in vivo in the injured or diseased brain is largely unknown. In this study, we utilized magnetic resonance imaging (MRI) techniques in order to understand the fates of human NSCs (HB1.F3) following transplantation into a rodent model of MCAo.
METHODS AND RESULTS
HB1.F3 human NSCs were pre-labeled with ferumoxides (Feridex(R))-protamine sulfate complexes, which were visualized and examined by MRI up to 9 weeks after transplantation. Migration of the transplanted cells to the infarct area was further confirmed by histological methods.
CONCLUSIONS
Based on these observations, we speculate that the transplanted NSCs have the extensive migratory ability to the injured site, which will in turn contribute to functional recovery in stroke.

Keyword

Human neural stem cells (hNSCs); Ischemic stroke; Middle cerebral artery occlusion (MCAo); Magnetic resonance imaging (MRI); Feridex; In vivo tracking

MeSH Terms

Brain
Dextrans
Humans
Infarction, Middle Cerebral Artery
Magnetic Resonance Imaging
Magnetite Nanoparticles
Models, Animal
Neural Stem Cells
Rodentia
Stroke
Track and Field
Transplants
Dextrans
Magnetite Nanoparticles