Korean J Parasitol.  2012 Jun;50(2):181-183. 10.3347/kjp.2012.50.2.181.

Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity

• Yang HJ

• KMID: 1384751
• DOI: http://doi.org/10.3347/kjp.2012.50.2.181

Abstract

The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

Keyword

Spirometra mansoni; sparganum; tegument; transmission electron microscopy (TEM); immunoblot

MeSH Terms

Animal Structures/immunology/ultrastructure
Animals
Antigens, Helminth/chemistry/*immunology/isolation & purification
Helminth Proteins/chemistry/*immunology/isolation & purification
Humans
Immunoblotting
Mice
Mice, Inbred BALB C
Microscopy, Electron
Molecular Weight
Sparganum/*immunology/*ultrastructure