- The Role of the Signal-to-Cutoff Ratio in Automated Anti-HCV Chemiluminescent Immunoassays by Referring to the Nucleic Acid Amplification Test and the Recombinant Immunoblot Assay
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Choi MS, Lee K, Hong YJ, Song EY, Kim DS, Song J
- KMID: 2434737
- Ann Lab Med.
- 2018 Sep;38(5):466-472.
- doi: 10.3343/alm.2018.38.5.466
BACKGROUND: Following discontinuation of the recombinant immunoblot assay (RIBA), the only available supplementary test for the detection of hepatitis C virus (HCV) is the nucleic acid amplification test (NAAT). However,... -
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- Helicobacter pylori Antigens Inducing Early Immune Response in Infants
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Seo JH, Youn JH, Kim EA, Jun JS, Park JS, Yeom JS, Lim JY, Woo HO, Youn HS, Ko GH, Park JS, Baik SC, Lee WK, Cho MJ, Rhee KH
- KMID: 2379609
- J Korean Med Sci.
- 2017 Jul;32(7):1139-1146.
- doi: 10.3346/jkms.2017.32.7.1139
To identify the Helicobacter pylori antigens operating during early infection in sera from infected infants using proteomics and immunoblot analysis. Two-dimensional (2D) large and small gel electrophoresis was performed using... -
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- Consideration of Appropriateness of Application of Immunoblot Assay as a Reentry Test for HCV or HIV Screening Reactive Donors
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Kang JW, Youn KW, Seo JH, Seo YI, Park S
- KMID: 2368790
- Korean J Blood Transfus.
- 2016 Dec;27(3):274-284.
- doi: 10.17945/kjbt.2016.27.3.274
BACKGROUND: Currently, serological assay, immunoblotting, and nucleic acid amplification test (NAT) are required as reentry tests for deferred donors with hepatitis C virus (HCV) or human immunodeficiency virus (HIV) screening... -
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- Evaluation of the VIDAS Anti-HCV Assay for Detection of Hepatitis C Virus Infection
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Hyun J, Ko DH, Kang HJ, Whang DH, Cha YJ, Kim HS
- KMID: 2373593
- Ann Lab Med.
- 2016 Nov;36(6):550-554.
- doi: 10.3343/alm.2016.36.6.550
BACKGROUND: Anti-hepatitis C virus antibody (anti-HCV) assays are recommended for screening HCV-infected persons. The VIDAS Anti-HCV Assay (bioMérieux, France), based on the enzyme-linked fluorescence test principle, was recently introduced in... -
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- A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
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Khammari I, Saghrouni , Lakhal S, Bouratbine A, Ben Said M, Boukadida
- KMID: 1792214
- Korean J Parasitol.
- 2014 Oct;52(5):493-499.
- doi: 10.3347/kjp.2014.52.5.493
The determination of the accurate immune status of pregnant women is crucial in order to prevent congenital toxoplasmosis. Equivocal results with conventional serological techniques are not uncommon when IgG titers... -
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- Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes
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Han ET, Yang HJ, Park YJ, Park JH, Chai JY
- KMID: 1792170
- Korean J Parasitol.
- 2014 Apr;52(2):201-204.
This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity... -
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- Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand
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Kitkhuandee A, Munkong W, Sawanyawisuth K, Janwan P, Maleewong , Intapan PM
- KMID: 1712234
- Korean J Parasitol.
- 2013 Dec;51(6):755-757.
- doi: 10.3347/kjp.2013.51.6.755
Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the... -
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- A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis
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Janwan P, Intapan PM, Yamasaki H, Laummaunwai P, Sawanyawisuth K, Wongkham C, Tayapiwatana C, Kitkhuandee A, Lulitanond V, Nawa Y, Maleewong
- KMID: 1712233
- Korean J Parasitol.
- 2013 Dec;51(6):751-754.
- doi: 10.3347/kjp.2013.51.6.751
Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components... -
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- Identification of immunoglobulin E binding components of two major tree pollens, birch and alder
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Yoon MG, Kim MA, Jin HJ, Shin YS, Park HS
- KMID: 2262963
- Allergy Asthma Respir Dis.
- 2013 Sep;1(3):216-220.
- doi: 10.4168/aard.2013.1.3.216
PURPOSE: Pollinosis is one of the major allergic diseases caused by airborne pollens. Alder and birch pollens are the major sensitizing tree pollens in this country. The immunoglobulin E (IgE)... -
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- Application of bispecific antibody against antigen and hapten for immunodetection and immunopurification
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Kim H, Park S, Lee HK, Chung J
- KMID: 2154262
- Exp Mol Med.
- 2013 Sep;45(9):e43.
We present a bispecific antibody that recognizes an antigen and a hapten and can be applied to various biological assays, including immunoblotting and immunoprecipitation. In immunoblot analysis of serum, an... -
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- IgG Western Blot for Confirmatory Diagnosis of Equivocal Cases of Toxoplasmosis by EIA-IgG and Fluorescent Antibody Test
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Khammari I, Saghrouni , Yaacoub A, Gaied Meksi S, Ach H, Garma L, Fathallah , Ben Said M
- KMID: 1715698
- Korean J Parasitol.
- 2013 Aug;51(4):485-488.
- doi: 10.3347/kjp.2013.51.4.485
The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo... -
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- Cotinine-conjugated aptamer/anti-cotinine antibody complexes as a novel affinity unit for use in biological assays
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Park S, Hwang D, Chung J
- KMID: 1389735
- Exp Mol Med.
- 2012 Sep;44(9):554-561.
Aptamers are synthetic, relatively short (e.g., 20-80 bases) RNA or ssDNA oligonucleotides that can bind targets with high affinity and specificity, similar to antibodies, because they can fold into unique,... -
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- Comparison of Four Commercial ELISA Kits and In-House Immunoblotting for Diagnosis of Helicobacter pylori Infection
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Jeong HL, Jung YS, Jun JS, Yeom JS, Park JS, Seo JH, Lim JY, Park CH, Woo HO, Youn HS, Ko GH, Baik SC, Lee WK, Cho MJ, Rhee KH
- KMID: 2315325
- Pediatr Gastroenterol Hepatol Nutr.
- 2012 Jun;15(2):85-90.
PURPOSE: Commercial enzyme-linked immunosorbent assay (ELISA) kits have been considered less reliable for children than for adults. The aim of this study was to compare four ELISA kits and in-house... -
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- Electron Microscopy of the Separated Outer Tegument of the Sparganum and Its Antigenicity
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Yang HJ
- KMID: 1384751
- Korean J Parasitol.
- 2012 Jun;50(2):181-183.
- doi: 10.3347/kjp.2012.50.2.181
The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument... -
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- Serological Detection of Lyme Borreliosis Agents in Patients From Korea, 2005–2009
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Park SH, Hwang KJ, Chu H, Park MY
- KMID: 2517961
- Osong Public Health Res Perspect.
- 2011 Jun;2(1):29-33.
- doi: 10.1016/j.phrp.2011.04.004
Objectives Laboratory tests are now being used to identify seropositive cases in patients suspected of having a Lyme borreliosis (LB) infection. From 2005 to 2009, we analyzed the serological and epidemiological... -
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- Analysis of the Effectiveness of Blotting Assays for Donor Blood Screening
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Oh DJ, Kang JW, Park YM
- KMID: 2300009
- Korean J Blood Transfus.
- 2011 Apr;22(1):1-8.
BACKGROUND: Immunoblot assays (IBAs) have been widely used to confirm the reactivity of immunoassay. However, indeterminate (ID) results have shown the limits for interpreting IBAs. There is some debate about... -
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- Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay
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Yang JY, Oh EJ, Kim Y, Park YJ
- KMID: 1781676
- Korean J Lab Med.
- 2010 Dec;30(6):675-684.
- doi: 10.3343/kjlm.2010.30.6.675
BACKGROUND: Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory... -
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- Analysis of the Results in Recent 10-year Allergen Test about Patients with Urticaria
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Lee GY, Choi HY, Myung KB, Choi YW
- KMID: 2283942
- Ewha Med J.
- 2010 Sep;33(2):71-80.
- doi: 10.12771/emj.2010.33.2.71
OBJECTIVES: Urticaria is multifactorial disease. Type 1 hypersensitivity reaction plays an important role in developing or aggravating the disease, so determining of the causative allergens and avoiding them from patient's... -
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- Immunoblot Patterns of Taenia asiatica Taeniasis
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Jeon HK, Eom KS
- KMID: 1720614
- Korean J Parasitol.
- 2009 Mar;47(1):73-77.
- doi: 10.3347/kjp.2009.47.1.73
Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight... -
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- Two Cases of Anti-p200 Pemphigoid
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Kim MR, Chang JY, Kim SC
- KMID: 2248151
- Korean J Dermatol.
- 2008 Jun;46(6):821-825.
Anti-p200 pemphigoid is a recently defined subepidermal immunobullous disease. Most anti-p200 pemphigoid patients show the clinical features of bullous pemphigoid, dermatitis herpetiformis and linear IgA disease. It is characterized by... -
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