J Vet Sci.  2012 Mar;13(1):59-65. 10.4142/jvs.2012.13.1.59.

Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors

• Blanc AM
• Berois MB
• Tome LM
• Epstein AL
• Arbiza JR

• KMID: 1365004
• DOI: http://doi.org/10.4142/jvs.2012.13.1.59

Abstract

Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.

Keyword

antibody response; BHV-1 gD; HSV-1 amplicon vectors; immunization

MeSH Terms

Animals
Antibodies, Viral/blood
Blotting, Western/veterinary
Cattle
Female
Genetic Vectors/*immunology
Herpesvirus 1, Bovine/genetics/*immunology
Herpesvirus 1, Human/genetics/*immunology
Immunity, Humoral/immunology
Immunization/methods/veterinary
Infectious Bovine Rhinotracheitis/*immunology/prevention & control/virology
Mice
Mice, Inbred BALB C
Neutralization Tests/veterinary
Specific Pathogen-Free Organisms
Viral Proteins/genetics/*immunology
Viral Vaccines/immunology