Yonsei Med J.  1989 Sep;30(3):219-234. 10.3349/ymj.1989.30.3.219.

Tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenz[a]anthracene

Affiliations
  • 1Department of Biochemistry, Yonsei University Wonju College of Medicinene, Wonju, Korea.
  • 2Department of Biochemistry, Yonsei University College of Medicine, Seoul, Korea.

Abstract

The incidence of tumor and the time of expression, cellular localization and the molecular weight of tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenz[a]anthracene (DMBA) with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA) were studied. The time of the development of skin tumors in 0.1% DMBA-TPA treated rats was significantly shorter than that in rats which were treated with DMBA alone. In the complete carcinogenesis case, papillomas developed more slowly and were less common and also squamous cell carcinomas appeared much later. From the analysis of the proteins of each experimental group by SDS-PAGE and two dimensional gel electrophoresis, at least three tumor associated proteins were identified (54kd, pl = 5.66; 27kd, pl = 5.85; 11kd, pl = 4.90). Also these proteins were found in rat dorsal skin from 14 days gestation to 21 days postpartum, and disappeared after 28 days. In conclusions, two stage skin carcinogenesis could be successfully demonstrated in Sprague-Dawley rats and abnormal proteins were produced in DMBA or DMBA-TPA induced skin tumor. The tumor associated proteins of skin tumor induced by DMBA or DMBA-TPA were appeared at the late initiation stage or early promotion stage, and they were localized in plasma membrane and were glycoproteins that are thought to be related to the epidermal differentiation process.

Keyword

7,12-dimethylbenz[a]anthracene; 12-0-tetradecanoyl-phorbol-13-acetate; complete carcinogenesis; two-stage carcinogenesis; epidermal differentiation; tumor associated protein

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene
Animal
Antigens, Surface/*analysis
Carcinoma, Squamous Cell/*analysis/chemically induced/pathology
Cell Membrane/metabolism
Comparative Study
Glycoproteins/analysis
Membrane Proteins/*analysis
Papilloma/*analysis/chemically induced/pathology
Rats
Rats, Inbred Strains
Skin Neoplasms/*analysis/chemically induced/pathology
Support, Non-U.S. Gov't
Tetradecanoylphorbol Acetate
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