J Korean Acad Oral Maxillofac Radiol.  1997 Aug;27(2):63-82.

Tumor-associated proteins in rat submandibular gland induced by DMBA and irradiation

• Oh SO
• Choi SC
• Park TW
• You DS

Abstract

This study was performed in order to identify changes of the plasma membrane proteins in rat submandibular gland tumors induced by 7,12-dimethylbenz[a]anthracene [DMBA] and X-irradiation. Two kinds of tumor associated membrane proteins (protein A and B) were isolated with 3 M KCl extraction from rat submandibular gland tumors induced by DMBA and X-irradiation. To identify their antigenicities, immunoelectrophoresis and double immunodiffusion was carried out with various proteins extracted from liver, heart, skin and pancreas of adult rats and from embryonic liver, heart and skin. The rabbit antisera against the protein A did not cross-react with any of the proteins extracted from the above mentioned tissues, suggesting that protein A might be tumor specific antigen. However, the rabbit antisera against protein B was precipitated with proteins extracted from the liver of adult and embryonic rats. Polyacrylamide gel electrophoresis of these two proteins (A and B) showed that protein A was a dimer with molecular weights of 69,000 and 35,000 dalton, whereas protein B was a monomer with molecular weight of 50,000 dalton.

Keyword

Irradiation; DMNA; Tumor associated membrane proteins; Immunoelectrophoresis; Double immunodiffusion; Tumor specific antigen

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene*
Adult
Animals
Cell Membrane
Electrophoresis, Polyacrylamide Gel
Heart
Humans
Immune Sera
Immunodiffusion
Immunoelectrophoresis
Liver
Membrane Proteins
Molecular Weight
Pancreas
Rats*
Skin
Staphylococcal Protein A
Submandibular Gland*
9,10-Dimethyl-1,2-benzanthracene
Immune Sera
Membrane Proteins
Staphylococcal Protein A