J Vet Sci.  2002 Sep;3(3):167-173.

Immunohistochemical Study of the Pancreatic Endocrine Cells in the BALB/c mice: An Unique Distributional Pattern of Glucagon

Affiliations
  • 1Pharmacology & Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharm. Ind. Co., Korea.
  • 2Department of Biology, Facult y of Natural Sciences, Kyungsan University, Korea. endohist@kyungsan.ac.kr
  • 3Department of Histology, College of Veterinary Medi cine,Kyungpook National University, Korea.

Abstract

The regional distribution and relative frequency of insulin-, glucagon-, somatostatin- and pancreatic polypeptide (PP)-producing endocrine cells in the pancreas of BALB/c mouse were investigated by immunohistochemical method. The pancreas of mice was divided into two portions; pancreatic islets and exocrine portions, and pancreatic islets were further subdivided into two regions (central and peripheral regions) and the relative frequency and regional distribution of immunoreactive cells against insulin, glucagon, somatostatin and PP antisera were monitored. In the pancreatic islet portions, insulin-immunoreactive cells were located in the central regions and they were randomly dispersed in the whole pancreatic islets in some case of the small islets. Quite different from those of other mammals, glucagon-immunoreactive cells were dispersed throughout central to peripheral regions in case of large islets and in the smaller ones, most of these cells were situated in the peripheral regions. Somatostatin-immunoreactive cells were detected in the peripheral regions with various frequencies. Although some cells were demonstrated in the central regions of pancreatic islets, most of PP-immunoreactive cells were located in the peripheral regions. In the exocrine portions, all four types of immunoreactive cells were demonstrated in the BALB/c mouse. Some peculiar distributional patterns of pancreatic endocrine cells were found in BALB/c mouse, especially in case of glucagon-immunoreactive cells.

Keyword

BALB/c mouse; pancreatic endocrine cell; immunohistochemistry

MeSH Terms

Animals
Female
Glucagon/*metabolism
Immunohistochemistry/veterinary
Insulin/metabolism
Islets of Langerhans/cytology/*metabolism
Male
Mice
Mice, Inbred BALB C/*metabolism
Pancreatic Polypeptide/metabolism
Somatostatin/metabolism
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