Yonsei Med J.  1993 Sep;34(3):248-257. 10.3349/ymj.1993.34.3.248.

Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens

• Yong TS
• Kim DS
• Lee SY
• Im KI
• Lee KY

• KMID: 1092418
• DOI: http://doi.org/10.3349/ymj.1993.34.3.248

Abstract

Specific serum IgE levels of Clonorchis sinensis in infected humans were measured by avidin-biotin ELISA, and allergens from C. sinensis were identified by immunoblot and autoradiography. Then, allergens fractionated by Sephadex G-200 gel filtration were analyzed, and cross-reactive allergenic components of C. sinensis reacted with paragonimiasis sera were revealed. Fourteen out of 15 C. sinensis egg-positives were found to be serum IgE positive (absorbance > 0.27). Of 14 IgE-reacting allergen bands visualized, major allergens of 66, 61.5, 45, 37, 28.5, 23.5 and 15.5 KD were recognized by more than 50% of the sera of infected humans. The considerable individual variations of IgE immune responses to C. sinensis allergenic components were also noticed. C. sinensis extract was separated into 5 fractions by Sephadex G-200 gel filtration. Seventy-four KD allergen was recognized in the first fraction, 50, 45, 37, 29.5 and 28.5 KD in the third, and 15.5 KD in the fourth. Cross-reactive allergens with sera of paragonimiasis cases were identified as 66, 45, 28.5, 13 and 7.5 KD.

Keyword

IgE; Clonorchis sinensis; allergen; immunoblot; autoradiography; cross-reaction

MeSH Terms

Allergens/*immunology
Animal
Antibodies, Helminth/*blood
Antigens, Helminth/*immunology
Clonorchiasis/*immunology
Clonorchis sinensis/*immunology
Cross Reactions
Human
Immunoglobulin E/*blood
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