J Korean Med Sci.  1999 Feb;14(1):52-56. 10.3346/jkms.1999.14.1.52.

Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat

Affiliations
  • 1Department of Internal Medicine, Institute of Digestive Disease and Nutrition, Korea University College of Medicine, Seoul.

Abstract

To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37 degrees C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4+/-0.5 microL/30 min, 0.58+/-0.05 microEq/30 min, and 214.0+/-26.1 microg/30 min to 51.6+/-3.7 microL/30 min, 1.52+/-0.11 microEq/30 min, and 569.8+/-128.9 microg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.

Keyword

Pancreatic function tests; Somatostain; Cysteamine; Rats

MeSH Terms

Animal
Cysteamine/pharmacology*
Hormone Antagonists/pharmacology*
Hormones, Synthetic/pharmacology
In Vitro
Male
Octreotide/pharmacology
Pancreas/secretion
Pancreas/drug effects*
Perfusion
Rats
Rats, Sprague-Dawley
Somatostatin/antagonists & inhibitors*
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