J Korean Acad Conserv Dent.  2002 Mar;27(2):183-195. 10.5395/JKACD.2002.27.2.183.

Cytotoxic effects of prevotella nigrescens on cultured cells

• Han JS
• Kim ES
• Lee SJ
• You YO
• Han KS
• Im MK

• KMID: 1987277
• DOI: http://doi.org/10.5395/JKACD.2002.27.2.183

Abstract

No abstract available.

MeSH Terms

Cells, Cultured
Prevotella
Prevotella nigrescens

Figure

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  Fig. 1 Agarose gel electrophoresis of PCR products with use of primers from the 16S rRNA gene of P. nigresences. Lane 1 shows 1kb DNA ladder. Lane 2 shows P. nigrescens ATCC 33563. Lanes 3 to 8 show clinical isolates of P. nigrescens; lane 3, 203; lane 4, 304; lane 5, 305; lane 6, 2202; lane 7, 2203;and lane 8, 2302.

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  Fig. 2 Silver-stained SDS-PAGE of LPS prepared from P. nigrescens. Lane 1 is size marker. Lane 2 shows P. nigrescens ATCC 33563. Lanes 3 to 8 show clinical isolates of P.nigrescens; lane 3, 203; lane 4, 304; lane 5, 305; lane 6, 2202; lane 7, 2203;and lane 8, 2302.

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  Fig. 3 MC3T3-E1 osteoblasts in control culture show normal morphogy (×400).

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  Fig. 4 MC3T3-E1 osteoblasts exposed to 100µl of bacterial growth supernatants from P. nigrescens ATCC type strain show deformation of cell morphology and display granulation of cells (×400).

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  Fig. 5 NIH3T3 fibroblasts in control culture show normal morpholgy (×400).

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  Fig. 6 NIH3T3 fibroblasts exposed to 100µl of bacterial growth supernatants from P. nigrescens ATCC type strain became rounded and lost intercellular connection (×400).

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  Fig. 7 Human gingival fibroblasts in control culture show typical spindle-shaped morphology (×400).

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  Fig. 8 Human gingival fibroblasts exposed to 2.5 mg/ml of LPS from clinical isolate 2302 show severe morphological alteration and cell aggregations (×400).

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