J Korean Acad Periodontol.
2005 Mar;35(1):21-30. 10.5051/jkape.2005.35.1.21.
Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival and periodontal ligament fibroblasts treated with lipopolysaccharide from Prevotella intermedia
- Affiliations
-
- 1Department of Periodontology, College of Dentistry, Pusan National University, Korea.
- 2Department of Life Science, College of Natural Science, Silla University, Korea.
- 3Department of Periodontology, College of Dentistry, Yonsei University, Korea.
- KMID: 1962390
- DOI: http://doi.org/10.5051/jkape.2005.35.1.21
Abstract
- Matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes and implicated in the remodeling and degradation of extracellular matrix under both physiological and pathological conditions. Connective tissue degradation in periodontal diseases is thought to be due to excessive MMP activities over their specific inhibitors. The effects of lipopolysaccharide (LPS) from Prevotella intermedia, one of the major putative pathogens of periodontitis, on the expression of mRNA for MMPs and tissue inhibitors of metalloproteinases (TIMPs) in human gingival and periodontal ligament fibroblasts were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNAs encoding MMP-1, -2, -3, -10, and -14 was increased in human gingival fibroblasts treated with P. intermedia LPS, whereas MMP-11 and TIMP-2 mRNA expression was decreased in these cells stimulated with LPS. P. intermedia LPS increased the MMP-1, -2, -10, -11, and -14 mRNA expression and decreased TIMP-1 and -2 mRNA expression in human periodontal ligament fibroblasts. These findings imply that P. intermedia LPS may play an important role in the connective tissue degradation in periodontitis.
Keyword
MeSH Terms
-
Connective Tissue
Extracellular Matrix
Fibroblasts*
Humans*
Matrix Metalloproteinases*
Metalloproteases*
Peptide Hydrolases
Periodontal Diseases
Periodontal Ligament*
Periodontitis
Prevotella intermedia*
Prevotella*
RNA, Messenger*
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinases
Metalloproteases
Peptide Hydrolases
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
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