Exp Mol Med.  1998 Jun;30(2):93-99.

Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells

• Puri N
• Saxena RK

Abstract

A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.

Keyword

MHC class I; IFN-g; MHC-AF; Tumor cells; Cytokines

MeSH Terms

Animal
Antibodies/pharmacology
Chymotrypsin/metabolism
Chymotrypsin/chemistry
Comparative Study
Concanavalin A/pharmacology
Heat
Histocompatibility Antigens Class I/metabolism*
Histocompatibility Antigens Class I/drug effects
Interferon Type II/pharmacology
Interferon Type II/metabolism
Interferon Type II/immunology
Lymphocytes/physiology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Proteins/pharmacology*
Proteins/metabolism
Proteins/isolation & purification*
Spleen/cytology
Trypsin/metabolism
Trypsin/chemistry
Tumor Cells, Cultured/immunology
Tumor Cells, Cultured/drug effects