Tissue Eng Regen Med.  2023 Jun;20(3):447-459. 10.1007/s13770-023-00531-2.

Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

• Zhang Y
• Wang P
• Jin Mx
• Zhou Yq
• Ye L
• Zhu Xj
• Li Hf
• Zhou M
• Li Y
• Li S
• Liang Ky
• Wang Y
• Gao Y
• Pan Mx
• Zhou Sq
• Peng Q

• KMID: 2542962
• DOI: http://doi.org/10.1007/s13770-023-00531-2

Abstract

BACKGROUND
Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs.
METHODS
hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 °C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated.
RESULTS
SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling.
CONCLUSION
These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Keyword

Schisandrin B; Stem cells; Low temperature preservation