Immune Netw.  2020 Aug;20(4):e35. 10.4110/in.2020.20.e35.

Virus-like Particle (VLP) Mediated Antigen Delivery as a Sensitization Tool of Experimental Allergy Mouse Models

Affiliations
  • 1Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea
  • 2National Institute of Crop Science, Rural Development Administration (RDA), Wanju 55365, Korea
  • 3Department of Medicine, Seoul National University College of Medicine, Seoul 03080, Korea
  • 4Transplant Research Institute, Seoul National University Hospital, Seoul 03080, Korea

Abstract

Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses in vivo. Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Viruslike particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.

Keyword

Antigen delivery; Virus-like particles (VLPs); Antigen specific antibody responses; IgE; Allergic reacti
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