J Nutr Health.  2019 Jun;52(3):250-257. 10.4163/jnh.2019.52.3.250.

Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena

Affiliations
  • 1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumsung, Chungbuk 27709, Korea. seokh@korea.kr
  • 2Department of Food Science and Biotechnology, Chungbuk National University, Cheongju, Chungbuk 28644, Korea.

Abstract

PURPOSE
Aster glehnii (AG) and Aster yomena (AY) are medicinal plants that belong to the family Compositea and grow widely in Korea. Plants in the genus Aster have been used to treat snakebite wounds or bruises in oriental medicine. This study compared the effects of anti-oxidants and anti-adipocyte differentiation according to the species (the aerial parts of AG and AY).
METHODS
AG and AY were extracted using 70% ethanol (−E) and water (−W) at room temperature. The anti-oxidant activities were measured by total phenol contents (TPC), total flavonoid contents (TFC), DPPH and ABTS+ assay. In addition, correlation analysis was performed for the anti-oxidant compounds and effect. The level of anti-adipocyte differentiation was assessed using an oil red O assay on pre-adipocytes.
RESULTS
AG-W showed higher TPC (6.92 µg/mL) and AG-E presented higher TFC (8.22 µg/mL) than the other extracts. Furthermore, AG-E exhibited higher radical scavenging activity in the DPPH and ABTS+ assay (IC50: 104.88 and 30.06 µg/mL). In the cytotoxicity assay, AG and AY extracts at concentrations less than 100µg/mL were non toxic. AG-W reduced the lipid accumulation of 3T3-L1 cells significantly after differentiation (70.49%) compared to the other extracts.
CONCLUSION
These results show that the water extract of AG has anti-oxidant effects and reduces the differentiation of 3T3-L1 cells. Therefore, AG has utility as a functional food material for its anti-oxidant activities and ability to prevent lipid accumulation.

Keyword

anti-obesity; radical scavenging activity; Aster glehui; adipocyte
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