Biomol Ther.  2019 Jan;27(1):78-84. 10.4062/biomolther.2018.110.

Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking

Affiliations
  • 1Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Republic of Korea. chshpark@hanyang.ac.kr
  • 2Hanyang Biomedical Research Institute, Hanyang University, Seoul 04763, Republic of Korea.
  • 3Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. srcho918@yuhs.ac
  • 4Graduate Program of NanoScience and Technology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • 5Department of Microbiology, College of Medicine, Hanyang University, Seoul 04763, Republic of Korea.
  • 6Department of New Drug Discovery & Development, Graduate School of New Drug Discovery & Development, Chungnam National University, Daejeon 34134, Republic of Korea.

Abstract

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5"² UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

Keyword

Neuron specific; Surface antigen; Differentiation; In vivo monitoring

MeSH Terms

Antigens, Surface*
Brain Diseases
Neural Stem Cells
Neurons*
Stem Cells
Antigens, Surface
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