J Korean Orthop Assoc.  1996 Apr;31(2):388-394. 10.4055/jkoa.1996.31.2.388.

Hydrogen Peroxide Production in Neutrophils after Tourniquet Release

• Park IH
• Lee KB
• Song KW
• Lee JY
• Chun JW

• KMID: 2423620
• DOI: http://doi.org/10.4055/jkoa.1996.31.2.388

Abstract

The use of lower extremity tourniquets for procedures of the lower leg is considered routine in orthopedic surgery, but, lower extremity tourniquets do harm occasionally. While the tourniquet is inflated, metabolic changes such as increased PaCO2 , lactic acid, and serum potassium and decreased level of PaO2 and pH occur in the ischemic limb. Deflation of tourniquet results in release of anaerobic metabolic products during ischemia into systemic circulation. In this ischemia/reperfusion situation, oxygen free radicals could potentially be produced during the reperfusion period by several mechanisms. One of these mechanisms is release of intracellular superoxide or hydrogen peroxide by activated neutrophils in the area. These reactive oxygen species(ROS) could be a causative factor for the postreperfusion no-flow, lung injury, induction of tourniquet shock, etc. The purpose of this clinical study was to investigate the effect of tourniquet deflation on the hemodynamic changes, changes of blood gas analysis, and hydrogen peroxide production using flow cytometric analysis of fluorescent DCF(Dichlorofluorescein). Quantitative analysis of fluorescent DCF was performed in resting and fMLP(N-formyl-methyonyl-leucyl-phenylalanine) or PMA(phorbol myristate acetate) stimulated neutrophils. Also differences of these factors between two groups of tourniquet time, one is less than one hour and the other more than one to two hours, were analysed. The hemodynamics(blood pressure, pulse rate), arterial PO2, bicarbonate, base excess, and hydrogen peroxide production showed no significant change before and after tourniquet release(p>0.05). Arterial pH and PaCO2 decreased significantly until 10 and 5 minutes after tourniquet release, respectively(p>0.05). Tourniquet time didn't reveal any significances differences. These results indicate that tourniquet application with400mmHg pressure and less than 2 hours does not release significant hydrogen peroxide into systemic circulation during reperfusion period after tourniquet release.

Keyword

Tourniquet; Hydrogen Peroxide; Flow Cytometry

MeSH Terms

Blood Gas Analysis
Blood Pressure
Clinical Study
Extremities
Flow Cytometry
Free Radicals
Hemodynamics
Hydrogen Peroxide*
Hydrogen*
Hydrogen-Ion Concentration
Ischemia
Lactic Acid
Leg
Lower Extremity
Lung Injury
Myristic Acid
Neutrophils*
Orthopedics
Oxygen
Potassium
Reperfusion
Shock
Superoxides
Tourniquets*
Free Radicals
Hydrogen
Hydrogen Peroxide
Lactic Acid
Myristic Acid
Oxygen
Potassium
Superoxides

Figure

• 

  Fig. 1 Time course of increasing fluorescent DCF in resting and stimulated PMNL(poly morphonuclear leukocyte), this figure shows there is no difference of hydrogen per oxide production expressed as fluorescent DCF before and after tourniquetrelease. fMLP(10-8 M/L):N-formyl-methyonyl-luecyl-phenylala nine PMA(300 ng/ml/): Phorbol myritate acetate

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