Knee Surg Relat Res.
2018 Sep;30(3):193-205. 10.5792/ksrr.17.067.
Mesenchymal Stem Cells Isolated from the Anterior Cruciate Ligament: Characterization and Comparison of Cells from Young and Old Donors
- Affiliations
-
- 1Department of Orthopaedic Surgery, Koenig-Ludwig-Haus, Center for Musculoskeletal Research, Julius-Maximilians-University Wuerzburg, Wuerzburg, Germany. christian.konrads@gmail.com
- 2BioChip Laboratory, Center for Medical Biotechnology, University of Duisburg-Essen, Essen, Germany.
- 3Department of Orthopaedic Surgery, Harvard Medical School, Boston, MA, USA.
- 4Department of Orthopaedic Trauma, Center for Trauma Research, BG Trauma Center Tuebingen, University of Tuebingen, Tuebingen, Germany.
- 5Department of Orthopaedics and Traumatology, Agatharied Hospital, Teaching Hospital of the University of Munich, Hausham, Germany.
- KMID: 2420345
- DOI: http://doi.org/10.5792/ksrr.17.067
Abstract
- PURPOSE
Mesenchymal stem cells (MSCs) isolated from the anterior cruciate ligament (ACL) share multiple characteristics of bone marrow-derived mesenchymal stem cells (BMSCs), allowing their use for regenerative therapies. Injuries to the ACL can affect people of all ages. This study assesses whether the regenerative potential of ACL-derived MSCs (ACL-MSCs) from old donors is as high as the potential of ACL-MSCs from young donors.
MATERIALS AND METHODS
ACL-MSCs were isolated from ACL tissues obtained from young and old donors at the time of ACL reconstruction or arthroplasty. Proliferative capacity, multilineage differentiation potential (chondrogenic, osteogenic, and adipogenic lineages), and transcriptome-wide gene expression were assessed and compared between young and old donors. BMSCs of middle-aged donors served as an additional comparator.
RESULTS
No substantial differences between ACL-MSCs from young and old donors were observed in their proliferative capacity and multilineage differentiation potential. The latter did not substantially differ between both ACL-MSC groups and BMSCs. Differential expression of genes related to the cytoskeleton and to protein dephosphorylation amongst other pathways was detected between ACL-MSCs from young and old donors.
CONCLUSIONS
Regenerative potential of ACL-MSCs from old donors was not substantially lower than that from young donors, suggesting that regenerative therapies of ACL tears are feasible in both age groups. In vivo studies of the effect of age on the efficacy of such therapies are needed.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Concise Review: Differentiation of Human Adult Stem Cells Into Hepatocyte-like Cells In vitro
- Ligament Reconstruction in Congenital Absence of the Anterior Cruciate Ligament: A Case Report
- Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells
- Stem cell properties of cells derived from canine periodontal ligament
- Mesenchymal Stem Cells Decrease Tunnel Widening of Anterior Cruciate Ligament Reconstruction in Rabbit Model
