Int J Stem Cells.  2019 Mar;12(1):162-169. 10.15283/ijsc18022.

Mesenchymal Stem Cells Decrease Tunnel Widening of Anterior Cruciate Ligament Reconstruction in Rabbit Model

Affiliations
  • 1Center for Joint Disease, Chonnam National University Hwasun Hospital, Hwasun, Korea. seonbell@chonnam.ac.kr
  • 2Department of Pathology, Chonnam National University Medical School, Gwangju, Korea.

Abstract

BACKGROUND AND OBJECTIVES
The study investigated the effect of mesenchymal stem cells (MSCs) or fibrin glue on tunnel widening after anterior cruciate ligament (ACL) reconstruction compared with biologic free control without any biologic agents in the rabbit model.
METHODS AND RESULTS
ACL reconstructions were performed in 18 New Zealand white rabbits. All animals were divided into 3 groups according to the following reconstruction conditions and euthanized 12 weeks postoperatively for radiologic and histologic analyses. Thirty-two knees (control group=10; fibrin group=11; MSCs group=11) were finally evaluated. On micro-CT scan, mean femoral tunnel widening on oblique-sagittal image was 0.7±0.4 mm in the control group, 0.22±0.1 mm in the fibrin group and 0.25±0.1 mm in the MSCs group (p=0.001). Fibrin group and MSCs group showed significant differences compared with control group (p=0.002, 0.002). Mean tibial tunnel widening on oblique-sagittal image was 0.76±0.5 mm, 0.27±0.1 mm and 0.29±0.2 mm in the control, fibrin and MSCs group. Fibrin and MSCs group showed significant differences compared with control group (p=0.017, 0.014). Hounsfield Units (HU) were not significantly different between 3 groups (p>0.05). Histological analysis revealed that the architecture of graft in the MSCs group featured hypercellularity and compact collagen deposit.
CONCLUSION
ACL reconstruction using MSCs seemed decrease tunnel widening in rabbit model. Further study with large animals is required to confirm efficacy on decreasing tunnel widening.

Keyword

Anterior cruciate ligament; Mesenchymal stem cell; Tunnel widening; Rabbit model

MeSH Terms

Animals
Anterior Cruciate Ligament Reconstruction*
Anterior Cruciate Ligament*
Biological Factors
Collagen
Fibrin
Fibrin Tissue Adhesive
Knee
Mesenchymal Stromal Cells*
Rabbits
Transplants
Biological Factors
Collagen
Fibrin
Fibrin Tissue Adhesive
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