J Vet Sci.  2017 Jun;18(2):201-207. 10.4142/jvs.2017.18.2.201.

Antigenic characterization of classical swine fever virus YC11WB isolates from wild boar

Affiliations
  • 1Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. andj67@korea.kr
  • 2Korea Pork Producers Association, Seoul 06643, Korea.

Abstract

Classical swine fever (CSF), a highly contagious disease that affects domestic pigs and wild boar, has serious economic implications. The present study examined the virulence and transmission of CSF virus strain YC11WB (isolated from a wild boar in 2011) in breeding wild boar. Virulence of strain YC11WB in domestic pigs was also examined. Based on the severe clinical signs and high mortality observed among breeding wild boar, the pathogenicity of strain YC11WB resembled that of typical acute CSF. Surprisingly, in contrast to strain SW03 (isolated from breeding pigs in 2003), strain YC11WB showed both acute and strong virulence in breeding pigs. None of three specific monoclonal antibodies (7F2, 7F83, and 6F65) raised against the B/C domain of the SW03 E2 protein bound to the B/C domain of strain YC11WB due to amino acid mutations (⁷²â°K→R and ⁷²³N→S) in the YC11WB E2 protein. Although strains YC11WB and SW03 belong to subgroup 2.1b, they had different mortality rates in breeding pigs. Thus, if breeding pigs have not developed protective immunity against CSF virus, they may be susceptible to strain YC11WB transmitted by wild boar, resulting in severe economic losses for the pig industry.

Keyword

B/C domain; Classical swine fever virus; Sus scrofa; virulence

MeSH Terms

Animals
Animals, Wild/immunology/virology
Antibodies, Monoclonal/immunology
Antigens, Viral/*immunology
Classical Swine Fever/*immunology/transmission/virology
Classical swine fever virus/*immunology/isolation & purification/pathogenicity
Cloning, Molecular
Real-Time Polymerase Chain Reaction/veterinary
Sus scrofa/*virology
Swine
Antibodies, Monoclonal
Antigens, Viral

Figure

  • Fig. 1 Predicted structure of glycoprotein E2, and amino acid sequence similarities between the B/C and D/A domains of classical swine fever virus (CSFV) strains SW03 and YC11WB. (A) The best RCSB Protein Data Bank template for strains SW03 and YC11WB was 2YQ2 (BVDV1 envelope glycoprotein E2). (B) Amino acid sequence similarity between the B/C and D/A domains of strains SW03 and YC11WB. The B/C and D/A domains are marked with a green bar and a pink bar, respectively. Three linear epitope determinants identified by using antibody epitope prediction programs are shown in gray. The epitope region binding the WH303 mAb is shown as a black box.


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