J Vet Sci.  2017 Sep;18(3):299-306. 10.4142/jvs.2017.18.3.299.

Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells

• Kim HR
• Lee J
• Byeon JS
• Gu NY
• Lee J
• Cho IS
• Cha SH

• KMID: 2412447
• DOI: http://doi.org/10.4142/jvs.2017.18.3.299

Abstract

Mesenchymal stem cells (MSCs) isolated from various tissues have been well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been fully documented. In this study, we conducted extensive characterization of feline adipose tissue-derived MSCs (fAD-MSCs). Study fAD-MSCs were individually isolated from the intra-abdominal adipose tissues of six felines. The expression levels of cell surface markers and pluripotent markers were evaluated. Next, proliferation capacity was analyzed by performing cumulative population doubling level (CPDL) and doubling time (DT) calculation assays. Differentiation potentials of fAD-MSCs into mesodermal cell lineages were analyzed by examining specific staining and molecular markers. All fAD-MSCs positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6), whereas DT increased after P3 to P4. Also, stem cell-specific pluripotent markers (Oct3/4, Nanog, and SSEA-4) were detected. Importantly, all fAD-MSCs demonstrated mesodermal differentiation capacity. These results suggest that fully characterized fAD-MSCs could be beneficial when considering the use of these cells in feline disease research.

Keyword

adipose tissue-derived mesenchymal stem cells; differentiation; feline; multipotent; proliferation

MeSH Terms

Abdomen
Adipose Tissue/*cytology
Animals
Biomarkers/metabolism
Cats/*anatomy & histology
Cell Differentiation
Cell Proliferation
Flow Cytometry/veterinary
Fluorescent Antibody Technique/veterinary
Mesenchymal Stromal Cells/*cytology/physiology
Multipotent Stem Cells/cytology/physiology
Biomarkers