Nat Prod Sci.  2015 Mar;21(1):42-48. 10.0000/nps.2015.21.1.42.

Phytochemical Analysis of the Phenolic Fat-Suppressing Substances in the Leaves of Lactuca raddeana in 3T3-L1 Adipocytes

• Nugroho A
• Choi JS
• An HJ
• Park HJ

• KMID: 2409598
• DOI: http://doi.org/10.0000/nps.2015.21.1.42

Abstract

Lactuca raddeana (Compositae) is used to treat obesity and complications due to diabetes. The five phenolic compounds including chlorogenic acid, chicoric acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, luteolin were qualitatively identified by LC-ESI-MS analysis. The contents were quantitatively determined by HPLC, under the condition of a Capcell Pak C18 column (5 microm, 250 mm x 4.6 mm i.d.) and a gradient elution of 0.05% trifluoroacetic acid (TFA) and 0.05% TFA in MeOH-H2O (60 : 40). The contents of chicoric acid (100.99 mg/g extract) and luteolin 7-O-glucoside (101. 69 mg/g extract) were high, while those of other three phenolic substances were very low. The 3T3-L1 adipocyte cells treated with chicoric acid and luteolin 7-O-glucuronide significantly suppressed the accumulation of fat, suggesting they are effective against obesity. Since high level of peroxynitrite (ONOO) causes cardiovascular disease in obese patients, its scavenging activity was also studied.

Keyword

Lactuca raddeana; Compositae; Chicoric acid; Peroxynitrite; HPLC; Adipocyte

MeSH Terms

Adipocytes*
Asteraceae
Cardiovascular Diseases
Chlorogenic Acid
Chromatography, High Pressure Liquid
Humans
Luteolin
Obesity
Peroxynitrous Acid
Phenol*
Trifluoroacetic Acid
Chlorogenic Acid
Luteolin
Peroxynitrous Acid
Phenol
Trifluoroacetic Acid