J Obes Metab Syndr.  2019 Jun;28(2):105-111. 10.7570/jomes.2019.28.2.105.

Zaluzanin C Inhibits Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes

Affiliations
  • 1Department of Food and Nutrition, Daegu University, Gyeongsan, Korea. kimyh0128@daegu.ac.kr
  • 2Research Institute of Anti-Aging, Daegu University, Gyeongsan, Korea.
  • 3Institute of Industrial and Technology, Daegu University, Gyeongsan, Korea.

Abstract

BACKGROUND
An excess storage of body fat causes obesity. Since obesity increases risk of chronic diseases, it is important to inhibit excessive storage of fat. Zaluzanin C is a sesquiterpene lactone isolated from Ainsliaea acerifolia. The aim of this study was to demonstrate the effect of zaluzanin C on differentiation of 3T3-L1 preadipocytes into mature adipocytes.
METHODS
The cytotoxicity of zaluzanin C and its effect on cell proliferation was determined. For the induction of adipocyte differentiation, 3T3-L1 preadipocytes were treated with differentiating medium containing 10 μg/mL insulin, 115 μg/mL methylisobutylxanthine, and 1 μM dexamethasone. Differentiated 3T3-L1 cells were subjected to Oil red O solution or used for Western blot analysis. Zaluzanin C was added to the cell culture medium at concentrations of 0, 1, 2.5, 5, and 10 μM.
RESULTS
Zaluzanin C did not inhibit cell proliferation and showed no cytotoxicity at 10 μM concentration in 3T3-L1 cells. Therefore, concentration range of 0-10 μM zaluzanin C was used for subsequent experiments. Zaluzanin C inhibited accumulation of lipid droplets in 3T3-L1 adipocytes. To understand the underlying mechanism of zaluzanin C, expression of adipogenesis regulators was determined by Western blot analysis. Zaluzanin C suppressed peroxisome proliferator-activated receptor gamma (PPARγ) expression, an adipogenesis related transcription factor, and inhibited aP2/fatty acid-binding protein-4 expression, a target gene of PPARγ. However, it did not affect expression of CCAAT/enhancer-binding protein alpha related with acquisition of insulin sensitivity.
CONCLUSION
These data suggest that inhibitory effect of zaluzanin C on adipogenesis of 3T3-L1 adipocytes could be partially caused by suppressing PPARγ.

Keyword

Zaluzanin C; Lactone; 3T3-L1 cell; Proliferator-activated receptor gamma
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