Nutr Res Pract.  2014 Feb;8(1):33-39.

Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

Affiliations
  • 1Department of Food & Nutrition, Mokpo National University, 1666 Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonnam 534-729, Korea. kha@mokpo.ac.kr
  • 2Jeonnam Biofood Technology Center, Naju, Korea.

Abstract

Obesity occurs when a person's calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes and the accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation and apoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated with DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 5 microg/ml insulin and 1 microM dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations and expressions of PPAR-gamma and C/EBPalpha in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c and FAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomace water extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of prevention or treatment strategy for obesity.

Keyword

Pear pomace water extract; 3T3-L1 cells; adipogenesis; apoptosis

MeSH Terms

1-Methyl-3-isobutylxanthine
3T3-L1 Cells
Adipocytes*
Adipogenesis*
Apoptosis*
Blotting, Western
Burns
Cell Survival
Dexamethasone
Insulin
Obesity
Pyrus*
Sterol Regulatory Element Binding Protein 1
Water*
1-Methyl-3-isobutylxanthine
Dexamethasone
Insulin
Sterol Regulatory Element Binding Protein 1
Water
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