Korean J Pediatr.
2012 Dec;55(12):474-480.
Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test
- Affiliations
-
- 1Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea. kdh281920@gmail.com
- 2Department of Pediatrics, Korea University College of Medicine, Seoul, Korea.
- 3Department of Pediatrics, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.
- 4Department of Pediatrics, Inje University Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Korea.
- 5Department of Pediatrics, Dongguk University Ilsan Hospital, Goyang, Korea.
- 6Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.
Abstract
- PURPOSE
For evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test to determine its usefulness as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.
METHODS
We compared the MN test with the HI test using 50 paired samples taken from a previous clinical study (2008-2009) in Korean children under 18 years of age.
RESULTS
The linear correlation coefficients of the 2 tests for H3N2, H1N1, and influenza B were 0.69, 0.70, and 0.66, respectively. We identified a high index of coincidence between the 2 tests. For an influenza vaccine, the postvaccination seroprotection rates and seroconversion rates determined by the MN test were 78.0% and 96.0%, 90% and 42.0%, and 42.0% and 48.0% for H3N2, H1N1, and influenza B, respectively. Geometric mean titer fold increases of H3N2, H1N1, and influenza B were 2.89, 5.04, and 4.29, respectively, and were 2.5-fold higher. We obtained good results in the evaluation of the immunogenicity of the 2008-2009 seasonal influenza vaccines.
CONCLUSION
We found that the MN test was as effective as the HI test. Therefore, we suggest that the MN test can be used as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Assessment of Influenza Vaccine Immunogenicity in Immunocompromized Host During 2009 Influenza Season: A Single Institution Experience
- The 2009 H1N1 Pandemic Influenza in Korea
- Seasonal influenza and vaccine herd effect
- Influenza
- Sleep and vaccine administration time as factors influencing vaccine immunogenicity
