Korean J Vet Res.  2017 Dec;57(4):215-222. 10.14405/kjvr.2017.57.4.215.

Temporal and subcellular distributions of Cy5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days as a drug carrier

Affiliations
  • 1College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130-118, China.
  • 2College of Veterinary Medicine and Veterinary Medical Center, Chungbuk National University, Cheongju 28644, Korea. ywyun@cbu.ac.kr, synam@cbu.ac.kr
  • 3Asan Institute for Life Sciences, Asan Medical Center and University of Ulsan, Seoul 05505, Korea.

Abstract

Temporal and subcellular distributions of hyaluronic acid (HA) as a degradable nanoparticle (NP) in animals were investigated to determine if HA-NP could be utilized as an appropriate drug delivery system. After mice were intravenously injected with 5 mg/kg of Cy5.5-labeled HA-NP sized 350-400 nm or larger HA-polymers, the fluorescence intensity was measured in all homogenized organs from 0.5 h to 28 days. HA-NP was greatly detected in spleen, liver and kidney until day 28, while it was maintained at low levels in other organs. HA-polymer was observed at low levels in all organs. HA-NP quantities in spleen and liver were reduced until day 3, but increased sharply between days 3 and 7, then decreased again, while their HA-polymers were maintained at low levels until day 28. In kidneys, both HA-NP and HA-polymer showed high levels after 0.5 h of administration, but steadily decreased until day 28. According to ultrastructural analyses, HA-NP was engulfed in Kupffer cells of liver and macrophages of spleen and kidney at day 1 and was accumulated in the cytoplasm of kidney tubular cells at day 7. Overall, these findings suggest that HA-NP could be considered a desirable drug carrier in the liver, kidney, or spleen.

Keyword

drug delivery systems; hyaluronic acid; nanoparticles; pharmacokinetics; subcellular localization
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