J Korean Ophthalmol Soc.  2017 Oct;58(10):1155-1159. 10.3341/jkos.2017.58.10.1155.

The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers

Affiliations
  • 1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
  • 2Catholic Union Eye Clinic, Daegu, Korea.

Abstract

PURPOSE
To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers.
METHODS
HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
RESULTS
Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05).
CONCLUSIONS
Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow.

Keyword

Bromfenac; Loteprednol; Permeability; Prednisolone; Trabecular meshwork cells

MeSH Terms

Anti-Inflammatory Agents*
Humans
Loteprednol Etabonate
Permeability*
Prednisolone
Trabecular Meshwork*
Anti-Inflammatory Agents
Loteprednol Etabonate
Prednisolone

Figure

  • Figure 1. Effect of bromfenac sodium (BF) and loteprednol etabonate (LE) on the survival of human trabecular meshwork cells in tissue culture. Both bromfenac sodium and loteprednol did not affect the survival of trabecular meshwork cells compared to non-exposed controls (X= 1/1,000 dilution).

  • Figure 2. Effect of prednisolone acetate (PD) on the survival of human trabecular meshwork cells in tissue culture. Exposure to 1/1,000 or 1/500 diluted prednisolone decreased the survival of trabecular meshwork cells compared to non-ex-posed controls (* p < 0.05) (X = 1/1,000 dilution).

  • Figure 3. Effects of bromfenac sodium (BF), loteprednol ta-bonate (LE), or prednisolone acetate (PD) on the permeability of carboxyfluorescin through the trabecular meshwork cell monolayer. Both loteprednol and prednisolone decreased the permeabilty of carboxyfluorescein significantly compared control using phosphated buffered saline (PBS) (* p < 0.05). Carboxyfluorescein intensity of outer chamber normalized to the mean value obtained using PBS (permeability 100%) (X= 1/1,000 dilution).


Reference

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