J Korean Ophthalmol Soc.  2015 Jan;56(1):104-108. 10.3341/jkos.2015.56.1.104.

Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells

• Kim BM
• Kim JW

• KMID: 2216161
• DOI: http://doi.org/10.3341/jkos.2015.56.1.104

Abstract

PURPOSE
To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers.
METHODS
HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities.
RESULTS
The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 microg/mL MMC. No effect on the permeability or cell survival was observed at 0.1 or 1.0 microm DEX (p > 0.05).
CONCLUSIONS
BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.

Keyword

Benzalkonium chloride; Carboxyfluorescein; Dexamethasone; Mitomycin C; Trabecular meshwork cells

MeSH Terms

Benzalkonium Compounds*
Cell Survival
Dexamethasone*
Humans
Mitomycin*
Permeability
Trabecular Meshwork*
Benzalkonium Compounds
Dexamethasone
Mitomycin