Lab Anim Res.  2016 Dec;32(4):187-193. 10.5625/lar.2016.32.4.187.

Retrospective growth kinetics and radiosensitivity analysis of various human xenograft models

Affiliations
  • 1Radiation Non-clinical Center, Seoul, Korea. amy3523@kirams.re.kr
  • 2Department of Radiation Oncology, Seoul, Korea. mskim@kirams.re.kr
  • 3Division of Heavy Ion Clinical Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
  • 4Department of Biosystems Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.

Abstract

The purpose of this study was to delineate the various factors that affect the growth characteristics of human cancer xenografts in nude mice and to reveal the relationship between the growth characteristics and radiosensitivity. We retrospectively analyzed 390 xenografts comprising nine different human cancer lines grown in nude mice used in our institute between 2009 and 2015. Tumor growth rate (TGR) was calculated using exponential growth equations. The relationship between the TGR of xenografts and the proliferation of the cells in vitro was examined. Additionally, we examined the correlations between the surviving fractions of cells after 2 Gy irradiation in vitro and the response of the xenograft to radiation. The TGR of xenografts was positively related to the proliferation of the cells in vitro (r(P)=0.9714, p<0.0001), whereas it was independent of the histological type of the xenografts. Radiation-induced suppression of the growth rate (T/C%) of xenografts was positively related to the radiosensitivity of the cells in vitro (SFâ‚‚; r(P)=0.8684, p=0.0284) and TGR (r(P)=0.7623, p=0.0780). The proliferation of human cancer cells in vitro and the growth rate of xenografts were positively related. The radiosensitivity of cancer cells, as judged from the SFâ‚‚ values in vitro, and the radiation-induced suppression of xenograft growth were positively related. In conclusion, the growth rate of human xenografts was independent of histological type and origin of the cancer cells, and was positively related to the proliferation of the cancer cells in vitro.

Keyword

Human xenograft model; retrospective analysis; radiation; cell line characteristics; in vitro-in vivo correlation

MeSH Terms

Animals
Heterografts*
Humans*
In Vitro Techniques
Kinetics*
Mice
Mice, Nude
Radiation Tolerance*
Retrospective Studies*
Full Text Links
  • LAR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error