J Korean Neurol Assoc.
2003 Oct;21(5):513-520.
Expression of Complement Regulator Genes in Abeta1-42 Stimulated Human Neuroblastoma Cell
- Affiliations
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- 1Department of Pathology, College of Medicine, Catholic University of Korea, Korea. complt@cmc.cuk.ac.kr
- 2Department of Neurology, College of Medicine, Catholic University of Korea, Korea.
Abstract
- BACKGROUND
Endogenous complement inhibitors in the brain may protect against the neuroinflammation in Alzheimer's disease. Human neuroblastoma cells were stimulated by Abeta1 - 4 2 to investigate whether the expression of various complement regulator genes is upregulated. METHODS: SK-N-SH cells were incubated overnight with a single dose of 20 microM of Abeta1-42 or 0.5 ng/ml - 5 ng/ml of TNFalpha or both. Actinomycin D (2.5 microM) or cycloheximide (2.5 microM) was also added to the cell suspension. Messenger RNA expression of decay accelerating factor (DAF), membrane cofactor protein (MCP), CD59, complement-receptor 1(CR1), C1 inhibitor (C1-INH), C4-binding protein, factor H, factor I, clusterin and S-protein was measured by RT-PCR. RESULTS: Abeta1-42 and TNFalpha upregulated the expression of C1- INH significantly but increased expression of mRNA for factor H was not statistically significant. The expression of mRNAs for DAF and MCP was at low a level after stimulation. Factor I, CD59 and clusterin were not changed in their mRNA level. The mRNAs for S-protein, C4-binding protein and CR1 were not detected. Actinomycin D suppressed mRNA levels of C1-INH and CD59 significantly. Cycloheximide also inhibited the expression of both C1-INH and CD59. CONCLUSIONS: Early upregulated expression of C1-INH in Abeta1-42 stimulated neuroblastoma cell may contribute to a host defense mechanism against complement-mediated neuronal cell damage.
Keyword
MeSH Terms
-
Alzheimer Disease
Amyloid beta-Peptides
Antigens, CD46
Antigens, CD55
Antigens, CD59
Brain
Clusterin
Complement Factor H
Complement System Proteins*
Cycloheximide
Dactinomycin
Fibrinogen
Genes, Regulator*
Humans*
Neuroblastoma*
Neurons
RNA, Messenger
Tumor Necrosis Factor-alpha
Amyloid beta-Peptides
Antigens, CD46
Antigens, CD55
Antigens, CD59
Clusterin
Complement Factor H
Complement System Proteins
Cycloheximide
Dactinomycin
Fibrinogen
RNA, Messenger
Tumor Necrosis Factor-alpha
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