J Korean Acad Periodontol.  2009 Aug;39(Suppl):223-230.

Bone regeneration capacity of two different macroporous biphasic calcium materials in rabbit calvarial defect

• Park JC
• Lim HC
• Sohn JY
• Yun JH
• Jung UW
• Kim CS
• Cho KS
• Kim CK
• Choi SH

Abstract

ABSTRACT PURPOSE: Synthetic bone products such as biphasic calcium phosphate (BCP) are mixtures of hydroxyapatite (HA) and a- tricalcium phosphate (a- TCP). In periodontal therapies and implant treatments, BCP provides to be a good bone reconstructive material since it has a similar chemical composition to biological bone apatites. The purpose of this study was to compare bone regeneration capacity of two commercially available BCP.
METHODS
Calvarial defects were prepared in sixteen 9-20 months old New Zealand White male rabbits. BCP with HA and a- TCP (70:30) and BCP with Silicon-substituted hydroxyapatite (Si-HA) and a-TCP (60:40) particles were filled in each defect. Control defects were filled with only blood clots. Animals were sacrificed at 4 and 8 week postoperatively. Histomorphometric analysis was performed.
RESULTS
BCP with HAand a- TCP 8 weeks group and BCP with Si-HA and a- TCP 4 and 8 weeks groups showed statistically significant in crease (P<0.05) in augmented area than control group. Newly formed bone area after 4 and 8 weeks was similar among all the groups. Residual materials were slightly more evident in BCP with HA and a- TCP 8 weeks group.
CONCLUSIONS
Based on histological results, BCP with HA and a- TCP and BCP with Si-HA and a- TCP appears to demonstrate acceptable space maintaining capacity and elicit significant new bone formation when compared to natural bone healing in 4 and 8 week periods.

Keyword

bone substitutes; hydroxyapatite wound healing

MeSH Terms

Animals
Apatites
Bone Regeneration
Bone Substitutes
Calcium
Calcium Phosphates
Durapatite
Humans
Hydroxyapatites
Male
New Zealand
Osteogenesis
Rabbits
Apatites
Bone Substitutes
Calcium
Calcium Phosphates
Durapatite
Hydroxyapatites

Figure

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  Figure 1 Schematicdrawing of designated material filled in defects. Dotted line represents sagittal suture of cranium; A: Control, B: Bonemedik-DM®, C: Osteon®.

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  Figure 2 Light micrographs of control group at 4 weeks postoperatively. Thick fibrous tissue is covering the defect ×6 (A), ×200 (B).

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  Figure 3 Light micrographs of control group at 8 weeks postoperatively. Mature bone tissue is observed among connective tissue ×16 (A), ×200 (B).

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  Figure 4 Light micrographs of Osteon group at 4 weeks postoperatively. Relatively large particle of residual materials are surrounded obsteoblasts and newly formed bone ×16 (A), ×200 (B).

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  Figure 5 Light micrographs of Osteon group at 8 weeks postoperatively. Relatively small particles are scattered and newly formed bone are surrounding ×16 (A), ×200 (B).

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  Figure 6 Light micrographs of Bonemedik-DM at 4 weeks postoperatively. Active resorption process is observed ×16 (A), ×200 (B).

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  Figure 7 Light micrographs of Bonemedik-DM at 8 weeks postoperatively. Mature bone tissue is apposed interspace of residual materials ×16 (A), ×200 (B).

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