Korean J Vet Res.  2014 Jun;54(2):91-99.

Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea

• Cho HH
• Kim SH
• Min W
• Ku BK
• Kim JH
• Kim YH

Abstract

An antimicrobial susceptibility test was conducted to compare the resistance rates among Campylobacter spp. isolates from dogs (n = 50) raised under diverse conditions and humans (n = 50). More than 60% of Campylobacter (C.) jejuni from dogs and humans showed resistance to nalidixic acid, enrofloxacin and ciprofloxacin. C. jejuni isolates from humans showed higher resistance to tetracycline (83.3%) and ampicillin (91.3%) than those from dogs. None of the C. jejuni or Campylobacter coli isolates from humans or dogs were resistant to erythromycin. Overall, 85% of Campylobacter spp. isolates showed a multidrug resistant phenotype. Nucleotide sequencing analysis of the gryA gene showed that 100% of NA(R)/CIP(R) C. jejuni isolates from dogs and humans had the Thr-86th-Ile mutation, which is associated with fluoroquinolone resistance. flaA PCR restriction fragment length polymorphism (RFLP) typing to differentiate the isolates below the species level revealed 12 different clusters out of 73 strains. The human isolates belonged to eight different RFLP clusters, while five clusters contained dog and human isolates.

Keyword

antibiotic resistance; Campylobacter spp.; DNA gyrase; point mutation; RFLP

MeSH Terms

Ampicillin
Animals
Campylobacter coli
Campylobacter*
Ciprofloxacin
DNA Gyrase
Dogs*
Drug Resistance, Microbial
Epidemiological Monitoring*
Erythromycin
Humans
Korea
Nalidixic Acid
Phenotype
Point Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length*
Tetracycline
Ampicillin
Ciprofloxacin
DNA Gyrase
Erythromycin
Nalidixic Acid
Tetracycline