Korean J Gastrointest Motil.  2003 Jun;9(1):53-58.

Altered Colonic Motor Functions in Experimental Colitis of Guinea Pigs

Affiliations
  • 1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. hjpark21@yumc.yonsei.ac.kr

Abstract

BACKGROUND/AIMS
Trinitrobenzene sulphonic acid (TNBS)-induced colonic inflammation in rat alters colonic motor function. The aims of this study were to investigate the effect of TNBS-induced colitis on the colonic motor function of a guinea pig along the course of colitis and to document persistently altered colonic motor function after resolution of inflammation.
METHODS
Colitis was induced in about 300 g male guinea pigs (Hartley) by intrarectal administration of 0.3 mL TNBS in 50% ethanol, while controls received 0.3 mL of 50% ethanol or not. After 24 hours, 48 hours, 72 hours, 1 week, and 2 weeks, the distal colon was taken for the investigation of gross and microscopic findings, muscle tension, and colonic transit.
RESULTS
Maximal mucosal injury and inflammation were evident from the 2nd day following the induction of colitis. Seven days after the induction of colitis, some portions of the damaged mucosa began to recover. Development of tension in response to carbachol was not altered significantly along the course of colitis. Colonic transit was delayed significantly at 3, 7, and 14 days after administration of TNBS.
CONCLUSIONS
Colonic transit in TNBS-induced colitis of guinea pigs is delayed, but not linearly related to the degree of inflammation. Delayed colonic transit is not related to the muscarinic receptor-mediated contractions of circular muscle from the inflamed colon. Further studies are required to determine the mechanism(s) involved in this motor change.

Keyword

Colitis; Trinitrobenzene sulphonic acid; Colonic motor function

MeSH Terms

Animals
Carbachol
Colitis*
Colon*
Ethanol
Guinea Pigs*
Guinea*
Humans
Inflammation
Male
Mucous Membrane
Muscle Tonus
Rats
Carbachol
Ethanol
Full Text Links
  • KJGM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr