Korean J Dermatol.  2012 Nov;50(11):959-968.

The Evaluation of Skin Safety and Skin Cell Toxicity for Scutellaria baicalensis Georgi Extract according to Extraction Conditions

Affiliations
  • 1Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea. beomjoon74@gmail.com
  • 2Functional Food & Nutrition Division, National Academy of Agricultural Science Rural Development Administration, Suwon, Korea.
  • 3P&K Skin Research Center, Seoul, Korea.
  • 4Daebong Life Science, Incheon, Korea.
  • 5Department of Dermatology, School of Medicine, Keimyung University, Daegu, Korea.

Abstract

BACKGROUND
Scutellaria baicalensis Georgi extract is used as a traditional herbal medicine. The efficacy of Scutellaria baicalensis Georgi extract is known for antioxidative activity, antiinflammation effect, antibacterial effect, inhibitory effect of melanin synthesis, sun protection effect, antiallergy effect, and etc.
OBJECTIVE
We confirmed the cell viability or inhibitory effect of melanin synthesis in HaCaT (human keratinocyte cell line) and B16F10 (murine melanoma cell line) cells and the skin safety test through a clinical test (dermal irritation study) for Scutellaria baicalensis Georgi extract, according to the extraction methods.
METHODS
We checked the cell viability, using MTT assay and inhibitory effect of melanin synthesis in B16F10 cells or HaCaT cells for thirty one Scutellaria baicalensis Georgi extract, according to the extraction methods. Then, we evaluated the skin safety for selected eight Scutellaria baicalensis Georgi extract through a primary dermal irritation test.
RESULTS
Among the thirty one Scutellaria baicalensis Georgi extracts, according to the extraction methods, we selected eight Scutellaria baicalensis Georgi extracts that were not detected with cell toxicity in HaCaT cells and B16F10 cells, and could have inhibited the melanin synthesis in B16F10 cells. The selected eight Scutellaria baicalensis Georgi extracts identified the skin safety through a primary dermal irritation test.
CONCLUSION
We expect clinical trials for whitening efficacy based on inhibitory effect of melanin synthesis and human skin safety for Scutellaria baicalensis Georgi extracts.

Keyword

Melanin synthesis; Primary dermal irritation test; Scutellaria baicalensis Georgi; Skin safety test
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