Korean J Urol.  1999 Jun;40(6):697-702.

The Study for Chromosome 3p Loss in Renal Cell Carcinoma by Fluorescence in Situ Hybridization Using Paraffin-Embedded Tissue

• Jeong H
• Jie JR
• Chung JS
• Kwak C
• Kim DY
• Lee SB
• Lee SE

Abstract

PURPOSE: Conventional pathologic classifications of human renal cell carcinoma give little insight into oncogenesis and little assistance in predicting the clinical behavior of this disease. For genetic classification, deletion of the short arm of chromosome 3(3p), the hallmark of nonpapillary/clear cell RCC, is a major diagnostic criterion. Because of the limited routine applicability of cytogenetics and molecular genetic techniques we investigated fluorescence in situ hybridization(FISH) for the detection of this aberration in RCC.
MATERIALS AND METHODS
Isolated nuclei from 8 human RCC paraffin embedded tissue sections were examined with a dual color FISH technique for loss of chromosome 3p. Telomeric DNA probe from 3p and an internal ploidy control probe, centromeric probe of chromosome 2, were applied to the isolated nuclei of RCC.
RESULTS
87.5% of the patients(7) lost chromosome 3p. The loss of 3p in the samples tested was unrelated to patient age, gender, tumor stage, and grade.
CONCLUSIONS
FISH for the detection of loss in 3p from paraffin embedded tissue sections provides a sensitive and feasible methods for the genetic classification of kidney tumors and FISH is a very useful diagnostic tool for detection of the genetic aberrations of the tumors.

Keyword

Renal cell carcinoma; Deletion of 3p; Fluorescence in situ hybridization

MeSH Terms

Arm
Carcinogenesis
Carcinoma, Renal Cell*
Chromosomes, Human, Pair 2
Classification
Cytogenetics
DNA
Fluorescence*
Humans
In Situ Hybridization*
In Situ Hybridization, Fluorescence
Kidney
Molecular Biology
Paraffin
Ploidies
DNA
Paraffin