Study on the Chromosome 13 and 17 of Prostatic Cancers by Fluorescence In Situ Hybridization Technique

Hyun, Jae Seog; Park, Jong Yoon

Abstract

To detect the numerical changes of the chromosome 13 and 17, containing tumor suppressor genes (retinoblastoma gene and p53) in normal tissue, intraepithelial neoplasm and carcinoma of the prostate, the Fluorescence In Situ Hybridization technique with paraffin embedded tissue was done. Materials observed in this study consisted of the 14 cases of localized carcinoma (T2aN 0M0) and 1 case of locally infiltrating cancer (T3aN2M0). The chromosomal number changes were counted at normal tissue, intraepithelial neoplasm and neoplastic gland at the same radical prostatectomy specimen. The results were as follows: 1. There were no significant chromosomal number changes between 13 and 17 in each group 2. The normal non-neoplastic duct, intraepithelial neoplasm and neoplastic gland of the experimental group showed no significant difference in chromosomal change. 3. There were significant changes more than 30% in chromosomal numbers between control Y chromosome and experimental chromosome 13 and 17 in each group. 4. With the results collected from this study, chromosomal aberration in number in prostate biopsy could represent the already established initiation of the cancerous change in prostate and the chromosomal aberration detection method would be a good early diagnostic modality to detect the high risk group who will develop the prostate cancer.