The Change of Serum Level of Xanthine Oxidase Activity, Glutathione and Malonyldialdehyde following Renal Ischemia/Reperfusion Injury in the Rat

Bae, In Soo; Jung, Hee Chang; Park, Tong Choon

Abstract

PURPOSE: It has been suggested in our previous study that the level of xanthine oxidase(XO) activity, glutathione(GSH) and malonydialdehyde(MDA) in renal tissue following renal ischemia/reperfusion(I/R) could be used as marker of oxidant stress. Present study was undertaken to investigate the serum level of XO activity. GSH and MDA following renal I/R and to elucidate potential use of the serum level of GS H and MDA as makers of renal function following I/R injury.
MATERIALS AND METHODS
Male Sprague-Dawley rats(200-250gm) were divided into 3 groups : group A - occlusion of bilateral renal arteries for 60 min, group B - pretreatment allopurinol(20mg/Kg). a radical scavenger, plus occlusion of renal arteries, and group C(control group) - sham operation. In group A and B, recirculation of renal arteries were performed for 30min. XO activity, xanthine dehydrogenase(XD) type conversion ratio, level of GSH and MDA were measured from venous blood.
RESULTS
Both XO activity(nM/mg/min) and XD type conversion ratio(%) were increased in group A(XO; 0.173+/-0.012, XD; 60.44+/-4.32) and decreased in group B(XO; 0.136+/-0.01, XD; 45.40+/-4.78) compared to control group(XO; 0.153+/-0.012, XD; 46.93+/- 3.45). The level of GSH(microM/g tissue), a scavenger of oxygen free radical(OFR), was also decreased in group A(0.130+/-0.021) compared to group B(0.179+/-0.021) and control group(0.186+/-0.017). In addition, the level of MDA(nM/g tissue), which is a stable end product of lipid peroxidation, was significantly increased in group A(0.076+/-0.006) compared to group B(0.057+/-0.005) and control group(0.053+/-0.004).
CONCLUSIONS
From these results, it is suggested that renal I/R injury is highly correlated with the production of OFR. Furthermore. the serum levels of MDA and GSH might be used as early markers of oxidant stress in association with renal I/R injury.